A naked-eye based strategy for semiquantitative immunochromatographic assay

文献类型： 外文期刊

第一作者： Zhang, Daohong

作者： Zhang, Daohong;Li, Peiwu;Zhang, Qi;Li, Ran;Ding, Xiaoxia;Zhang, Daohong;Li, Peiwu;Zhang, Wen;Ding, Xiaoxia;Zhang, Qi;Li, Ran;Zhang, Daohong;Li, Peiwu;Zhang, Wen;Li, Chang Ming

作者机构：

关键词： Semiquantitative;immunochromatographic assay;Naked-eye;On-site

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： It is critical to develop a cost-effective quantitative/semiquantitative assay for rapid diagnosis and on-site detection of toxic or harmful substances. Here, a naked-eye based semiquantitative immunochromatographic strip (NSI-strip) was developed, on which three test lines (TLs, TL~(-1), TL-II and TL-II1) were dispensed on a nitrocellulose membrane to form the test zone. Similar as the traditional strip assay for small molecule, the NSI-strip assay was also based on the competitive theory, difference was that the ana-lyte competed three times with the capture reagent for the limited number of antibody binding sites. After the assay, the number of TLs developed in the test zone was inversely proportional to the analyte concentration, thus analyte content levels could be determined by observing the appeared number of TLs. Taking aflatoxin B1 as the model analyte, visual detection limit of the NSI-strip was 0.06 ngmL~(-1) and threshold concentrations for TL-I-III were 0.125,0.5, and 2.0 ng mL~(-1), respectively. Therefore, according to the appeared number of TLs, the following concentration ranges would be detectable by visual examination: 0-0.06ngmL~(-1) (negative samples), and 0.06-0.125 ng mL~(-1), 0.125-0.5ngmL~(-1), 0.5-2.0ngm L~(-1) and

分类号： O65

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