Nitrate Reductase-Dependent Nitric Oxide Production Is Involved in Microcystin-LR-Induced Oxidative Stress in Brassica rapa

文献类型： 外文期刊

第一作者： Chen, Jian

作者： Chen, Jian;Zhong, You Ming;Zhang, Hai Qiang;Shi, Zhi Qi;Chen, Jian;Zhong, You Ming;Zhang, Hai Qiang;Shi, Zhi Qi;Chen, Jian;Zhong, You Ming;Zhang, Hai Qiang;Shi, Zhi Qi;Zhang, Hai Qiang

作者机构：

关键词： brassica rapa;microcystin-LR;nitrate reductase;nitric oxide;oxidative stress

期刊名称：WATER AIR AND SOIL POLLUTION （ 影响因子：2.52； 五年影响因子：2.574 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Histochemical and biochemical approaches were used to investigate the phytotoxicity induced by microcystin-LR (MC-LR) in the shoots of Brassica rapa seedlings. MC-LR exposure was able to induce oxidative stress by triggering the over-generation of reactive oxygen species (ROS) including superoxide anion radical (O_2·~-) and hydrogen peroxide (H_2O_2) in the shoots of B. rapa. MC-LR exposure led to the significant increase in the concentration of endogenous nitric oxide (NO) in B. rapa. However, such increase was completely suppressed by the treatment with nitrate reductase (NR) inhibitor NaN_3, while l-NMMA, a NO synthase (NOS) inhibitor, had only slight effect on the content of endogenous NO in MC-LR-treated plant. These data suggested that NR-dependent pathway was the main source for endogenous NO generation under MC-LR stress. Afterwards, treatment with NaN_3 reduced the ROS generation, lipid peroxidation, and loss of membrane integrity in MC-LR-treated plant. MC-LR stress induced the increase in the expression of superoxide dismutase, ascorbate peroxidase, and catalase. However, such an effect could be reversed by the treatment with NaN_3. These results indicate that NR-dependent NO production mediates MC-LR-induced oxidative stress by triggering the over-generation of ROS in B. rapa.

分类号： X

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