Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)
文献类型: 外文期刊
第一作者: Wang, Qingbiao
作者: Wang, Qingbiao;Zhang, Yangyong;Fang, Zhiyuan;Liu, Yumei;Yang, Limei;Zhuang, Mu
作者机构:
关键词: Cabbage;Cytoplasmic male sterility (CMS);Mitochondrial SSR (mtSSR);Chloroplast SSR (cpSSR)
期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )
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收录情况: SCI
摘要: The profiles of single sequence repeat (SSR) in six distinct allo-cytoplasmic male sterile (CMS) types of cabbage (Brassica oleracea L. var. capitata) were generated using 32 SSR primer pairs derived from the Arabidopsis thaliana chloroplast (cp) genome and another 21 SSR primers from the B. napus mitochondrial (mt) genome sequences. In total, 11 cpSSR and 4 mtSSR primers revealed polymorphism among the six cabbage CMS types, namely NigCMS, OguCMSR(1), OguCMSR(2), OguCMSR(3), OguCMSHY and PolCMS. Through cluster analysis, six cabbage CMS types could be unambiguously differentiated with just three sets of primers (ACP43, ACP47, mtSSR2). Analysis of the selected amplicon sequences showed high identity to that of the corresponding sequences in A. thaliana, B. rapa and B. napus. The aligned cluster analysis revealed that the polymorphism mainly included SSR number variation, single nucleotide polymorphism (SNP), and sequence insertion or deletion (InDel). Our results demonstrated that specific mitochondrial or chloroplast SSR analysis could be a feasible alternative means for cabbage CMS type identification.
分类号: Q94
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