Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)

文献类型: 外文期刊

第一作者: Wang, Qingbiao

作者: Wang, Qingbiao;Zhang, Yangyong;Fang, Zhiyuan;Liu, Yumei;Yang, Limei;Zhuang, Mu

作者机构:

关键词: Cabbage;Cytoplasmic male sterility (CMS);Mitochondrial SSR (mtSSR);Chloroplast SSR (cpSSR)

期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The profiles of single sequence repeat (SSR) in six distinct allo-cytoplasmic male sterile (CMS) types of cabbage (Brassica oleracea L. var. capitata) were generated using 32 SSR primer pairs derived from the Arabidopsis thaliana chloroplast (cp) genome and another 21 SSR primers from the B. napus mitochondrial (mt) genome sequences. In total, 11 cpSSR and 4 mtSSR primers revealed polymorphism among the six cabbage CMS types, namely NigCMS, OguCMSR(1), OguCMSR(2), OguCMSR(3), OguCMSHY and PolCMS. Through cluster analysis, six cabbage CMS types could be unambiguously differentiated with just three sets of primers (ACP43, ACP47, mtSSR2). Analysis of the selected amplicon sequences showed high identity to that of the corresponding sequences in A. thaliana, B. rapa and B. napus. The aligned cluster analysis revealed that the polymorphism mainly included SSR number variation, single nucleotide polymorphism (SNP), and sequence insertion or deletion (InDel). Our results demonstrated that specific mitochondrial or chloroplast SSR analysis could be a feasible alternative means for cabbage CMS type identification.

分类号: Q94

  • 相关文献

[1]Transcriptome Sequencing Analyses between the Cytoplasmic Male Sterile Line and Its Maintainer Line in Welsh Onion (Allium fistulosum L.). Liu, Qianchun,Wen, Changlong,Zhao, Hong,Wang, Jian,Wang, Yongqin,Lan, Yanping. 2016

[2]Workable male sterility systems for hybrid rice: Genetics, biochemistry, molecular biology, and utilization. Huang, Jian-Zhong,Zhang, Hua-Li,Shu, Qing-Yao,E, Zhi-Guo. 2014

[3]Candidate Reference Genes Selection and Application for RT-qPCR Analysis in Kenaf with Cytoplasmic Male Sterility Background. Chen, Peng,Khan, Aziz,Chen, Lihong,Liao, Xiaofang,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong,Liu, Dongmei. 2017

[4]A comparative study of the atp9 gene between a cytoplasmic male sterile line and its maintainer line and further development of a molecular marker specific for male sterile cytoplasm in kenaf (Hibiscus cannabinus L.). Chen, Peng,Liao, Xiaofang,Zhou, Bujin,Liao, Jian,Huang, Zhipeng,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong.

[5]Identification of seedling cabbages and weeds using hyperspectral imaging. Wei, Deng,Zhao Chunjiang,Xiu, Wang,Huang, Yanbo,Wei, Deng,Zhao Chunjiang,Xiu, Wang,Wei, Deng,Zhao Chunjiang,Xiu, Wang,Wei, Deng,Zhao Chunjiang,Xiu, Wang. 2015

[6]A comparison of the performance and biological efficiency of new knapsack sprayers and a controlled droplet application (CDA) sprayer for the control of the diamondback moth, Plutella xylostella L. (Lepidoptera : Plutellidae). Guo, SJ,Geitz, G,Franklin, T,Liu, YQ,Furlong, MJ,Zalucki, MP,Cooper, L. 2004

[7]Determination of sulforaphane in broccoli and cabbage by high-performance liquid chromatography. Liang, H,Yuan, QP,Dong, HR,Liu, YM. 2006

[8]The ability to manipulate plant glucosinolates and nutrients explains the better performance of Bemisia tabaci Middle East-Asia Minor 1 than Mediterranean on cabbage plants. Cui, Hongying,Guo, Litao,Wang, Shaoli,Xie, Wen,Wu, Qingjun,Zhang, Youjun,Jiao, Xiaoguo. 2017

[9]Enantioselective Residue Dissipation of Hexaconazole in Cucumber (Cucumis sativus L.), Head Cabbage (Brassica oleracea L. var. caulorapa DC.), and Soils. Wang, Xinquan,Zhang, Hu,Xu, Hao,Wang, Xiangyun,Wu, Changxing,Li, Zhen,Wang, Qiang,Yang, Hongda. 2012

[10]Genetic purity testing of F-1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata). Ye, Shan,Wang, Yan,Gong, Yiqin,Xu, Liang,Liu, Liwang,Huang, Danqiong,Li, Jianbin. 2013

[11]Residues and dissipation of chlorothalonil and azoxystrobin in cabbage under field conditions. Hou, Fan,Liu, Fengmao,Zhao, Liuwei.

[12]First Report of Root Rot Caused by Plectosphaerella cucumerina on Cabbage in China. Li, Pan-Liang,Chai, A-Li,Shi, Yan-Xia,Xie, Xue-Wen,Li, Bao-Ju.

[13]Reduction of Escherichia coli O157:H7 on produce by use of electrolyzed water under simulated food service operation conditions. Pangloli, Philipus,Hung, Yen-Con,Beuchat, Larry R.,Beuchat, Larry R.,King, C. Harold,Zha, Zhi-Hui.

[14]Valuable New Resistances Ensure Improved Management of Sclerotinia Stem Rot (Sclerotinia sclerotiorum) in Horticultural and Oilseed Brassica Species. You, Ming Pei,Uloth, Margaret B.,Barbetti, Martin J.,You, Ming Pei,Uloth, Margaret B.,Barbetti, Martin J.,You, Ming Pei,Barbetti, Martin J.,Li, Xi Xiang,Banga, Surinder S.,Banga, Shashi K..

[15]New host resistances to Pseudocercosporella capsellae and implications for white leaf spot management in Brassicaceae crops. Gunasinghe, Niroshini,You, Ming Pei,Barbetti, Martin J.,Barbetti, Martin J.,Li, Xi Xiang,Banga, Surinder S.,Banga, Shashi K..

[16]Hybrid lethality caused by two complementary dominant genes in cabbage (Brassica oleracea L.). Hu, Yang,Xue, Yu-qian,Liu, Ji-sheng,Fang, Zhi-yuan,Yang, Li-mei,Zhang, Yang-yong,Lv, Hong-hao,Liu, Yu-mei,Li, Zhan-sheng,Zhuang, Mu.

[17]Enantioselective degradation of indoxacarb in cabbage and soil under field conditions. Sun, Dali,Wu, Yijun,Liang, Hongwu,Li, Li,Qiu, Jing,Sun, Dali,Liu, Chenglan.

[18]Fine mapping of BoGL1, a gene controlling the glossy green trait in cabbage (Brassica oleracea L. Var. capitata). Liu, Dongming,Ye, Zhibiao,Liu, Dongming,Tang, Jun,Liu, Zezhou,Dong, Xin,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao,Sun, Peitian,Liu, Yumei,Li, Zhansheng,Fang, Zhiyuan,Yang, Limei.

[19]Photosynthetically active radiation determining yields for an intercrop of maize with cabbage. Wang, Qingsuo,Sun, Dongbao,Hao, Hong,Zhao, Xuejiao,Hao, Weiping,Liu, Qiong.

[20]Cgl2 plays an essential role in cuticular wax biosynthesis in cabbage (Brassica oleracea L. var. capitata). Liu, Dongming,Ye, Zhibiao,Liu, Dongming,Tang, Jun,Liu, Zezhou,Dong, Xin,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao,Sun, Peitian,Liu, Yumei,Li, Zhansheng,Fang, Zhiyuan,Yang, Limei. 2017

作者其他论文 更多>>