Development of a female-specific RAPD marker for Sargassum thunbergii gender identification using bulked segregant analysis

文献类型： 外文期刊

第一作者： Liu, Fuli

作者： Liu, Fuli;Sun, Xiutao;Wang, Wenjun;Liang, Zhourui;Wang, Feijiu

作者机构：

关键词： Sargassum thunbergii;Random amplified polymorphism DNA;Gender identification;Bulked segregant analysis

期刊名称：AQUATIC BOTANY （ 影响因子：2.473； 五年影响因子：2.417 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present paper, we report the development of a female-specific Random Amplified Polymorphism DNA (RAPD) marker for Sargassum thunbergii gender identification using bulked segregant analysis. Five male and five female mature individuals were selected as the samples, from which the genomic DNA were extracted and mixed to produce the male DNA pool and the female DNA pool respectively. The two DNA pools were subjected to RAPD analysis using a total of 100 decamer primers. Out of the 617 RAPD markers only one marker named F289 was polymorphic between the two DNA pools (only appeared in female DNA pool). Marker F289 was also only present in the five female individuals but absent in the five male individuals who made up the female and male DNA pool respectively. The female-specific character of marker F289 was further verified in the 12 male and 12 female individuals sampled randomly from the field. These results indicated that the marker F289 was a female-specific marker, which may link to the sex determination in S. thunbergii. Consequently, we concluded that F289 can be used as a reliable molecular marker for S. thunbergii gender identification. This marker will facilitate the artificial seedlings rearing based on sexual reproduction and also can be utilized in population genetics study as well as in sex-determining mechanism study for S. thunbergii

分类号： Q948.8

  • 相关文献

[1]Substituting seaweed with corn leaf in diet of sea cucumber (Apostichopus japonicus): Effects on growth, feed conversion ratio and feed digestibility. Wu, B.,Tan, J.,Yang, A.,Xia, S.,Fu, Z.,Rahman, M. M.,Rahman, M. M.,Rajkumar, M..

[2]Damage to the Membrane Permeability and Cell Death of Vibrio parahaemolyticus Caused by Phlorotannins with Low Molecular Weight from Sargassum thunbergii. Wei, Yuxi,Yu, Jia,Guo, Qi,Liu, Qi,Zhao, Ling,Xu, Chuanjin.

[3]Effect of phlorotannins from Sargassum thunbergii on blood lipids regulation in mice with high-fat diet. Wei, Yuxi,Xiao, Huihui,Zhao, Aiyun,Hu, Yingfen,Liu, Lei,Zhao, Ling,Xu, Chuanjin,Liu, Qi.

[4]Historical isolation and contemporary gene flow drive population diversity of the brown alga Sargassum thunbergii along the coast of China. Li, Jing-Jing,Hu, Zi-Min,Yao, Jian-Ting,Duan, De-Lin,Li, Jing-Jing,Hu, Zi-Min,Yao, Jian-Ting,Duan, De-Lin,Li, Jing-Jing,Sun, Zhong-Min,Liu, Fu-Li,Fresia, Pablo. 2017

[5]Two RAPD markers linked to a major fertility restorer gene in pepper. Zhang, BX,Huang, SW,Yang, GM,Guo, JZ. 2000

[6]Effect of the puroindoline locus and environment on Chinese fresh noodle texture. Yang, EN,Zou, YC,Chen, DS,Sheppard, J,Martin, D,Huang, S,Mares, D,Sutherland, MW. 2006

[7]Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Huang, XQ,Wang, LX,Xu, MX,Roder, MS. 2003

[8]Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis. Han, Xueying,Chen, Jianping,Han, Xueying,Yang, Yong,Wang, Xuming,Zhou, Jie,Yu, Chulang,Cheng, Chen,Cheng, Ye,Yan, Chengqi,Chen, Jianping,Zhang, Wenhao. 2014

[9]A Genome Scan for Quantitative Trait Loci Associated with Vibrio anguillarum Infection Resistance in Japanese Flounder (Paralichthys olivaceus) by Bulked Segregant Analysis. Wang, Lei,Wang, Lei,Fan, Caixia,Liu, Yang,Zhang, Yingping,Deng, Han,Xu, Ying,Tian, Yongsheng,Liao, Xiaolin,Xie, Mingshu,Li, Wenlong,Chen, Songlin,Wang, Lei,Liu, Shoutang,Sun, Deqiang.

[10]Bulked sample analysis in genetics, genomics and crop improvement. Zou, Cheng,Wang, Pingxi,Xu, Yunbi,Xu, Yunbi.

[11]QTL mapping of adult-plant resistance to stripe rust in a "Lumai 21xJingshuang 16' wheat population. He, Zhong H.,Xia, Xian C.,Ren, Yan,Liu, Li S.,He, Zhong H.,Wu, Ling,Bai, Bin.

[12]Genetic mapping of a putative Agropyron cristatum-derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array. Lu, Yuqing,Wu, Xiaoyang,Yao, Miaomiao,Zhang, Jinpeng,Liu, Weihua,Yang, Xinming,Li, Xiuquan,Du, Juan,Gao, Ainong,Li, Lihui.

[13]Isolation and identification of a gene in response to rice blast disease in rice. Zheng, XW,Chen, XW,Zhang, XH,Lin, ZZ,Shang, JJ,Xu, JC,Zhai, WX,Zhu, LH.

[14]Characterization and fine mapping of a novel barley Stage Green-Revertible Albino Gene (HvSGRA) by Bulked Segregant Analysis based on SSR assay and Specific Length Amplified Fragment Sequencing. Qin, Dandan,Dong, Jing,Xu, Fuchao,Ge, Shuangtao,Xu, Qing,Li, Meifang,Qin, Dandan,Dong, Jing,Xu, Fuchao,Ge, Shuangtao,Xu, Qing,Li, Meifang,Guo, Ganggang,Xu, Yuxin. 2015

[15]The earlier identification of the seedless characteristic of the wampee [Clausena lansium (Lour.) Skeels] hybrid by a random amplified polymorphic DNA (RAPD) marker. Zhao Zhichang,Hu Guibing,Ou Yangruo,Liu Yunchun,Zhao Zhichang,Yi Yang,Zhao Zhichang,Chen Yeyuan. 2010

[16]Development of Sequence Characterized Amplified Region (SCAR) Primers for the Detection of Resistance to Sporisorium reiliana in Maize. Shi Hong-liang,Zhang De-gui,Pan Guang-tang,Shi Hong-liang,Li Xin-hai,Xie Chuan-xiao,Hao Zhuan-fang,Li Ming-shun,Zhang Shi-huang. 2009

[17]Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus). Zhang, H.,Sun, R.,Zhang, H.,Strelkov, S. E.,Feng, J.,Hwang, S. -F.,Falak, I.,Huang, X..

作者其他论文 更多>>

微信小程序