Estimation of recombination frequency in bi-parental genetic populations

文献类型： 外文期刊

第一作者： Sun, Ziqi

作者： Sun, Ziqi;Li, Huihui;Zhang, Luyan;Wang, Jiankang

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期刊名称：GENETICS RESEARCH （ 影响因子：1.588； 五年影响因子：1.263 ）

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收录情况： SCI

摘要： Linkage analysis plays an important role in genetic studies. In linkage analysis, accurate estimation of recombination frequency is essential. Many bi-parental populations have been used, and determining an appropriate population is of great importance in precise recombination frequency. In this study, we investigated the estimation efficiency of recombination frequency in 12 bi-parental populations. The criteria that we used for comparison were LOD score in testing linkage relationship, deviation between estimated and real recombination frequency, standard error (SE) of estimates and the least theoretical population size (PS) required to observe at least one recombinant and to declare the statistically significant linkage relationship. Theoretical and simulation results indicated that larger PS and smaller recombination frequency resulted in higher LOD score and smaller deviation. Lower LOD score, higher deviation and higher SE for estimating the recombination frequency in the advanced backcrossing and selfing populations are larger than those in backcross and F-2 populations, respectively. For advanced backcrossing and selfing populations, larger populations were needed in order to observe at least one recombinant and to declare significant linkage. In comparison, in F-2 and F-3 populations higher LOD score, lower deviation and SE were observed for co-dominant markers. A much larger population was needed to observe at least one recombinant and to detect loose linkage for dominant and recessive markers. Therefore, advanced backcrossing and selfing populations had lower precision in estimating the recombination frequency. F-2 and F-3 populations together with co-dominant markers represent the ideal situation for linkage analysis and linkage map construction.

分类号： Q3

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