Development and Evaluation of a Reverse Transcription-Loop-Mediated Isothermal Amplification Method for Rapid Detection of Novel Duck Reovirus

文献类型： 外文期刊

第一作者： Li, Zhao-Long

作者： Li, Zhao-Long;Chen, Shi-Long;Chen, Shao-Ying;Lin, Feng-Qian;Cheng, Xiao-Xia;Wang, Shao;Zhu, Xiao-Li;Jiang, Bin;Li, Zhao-Long;Chen, Shi-Long;Chen, Shao-Ying;Lin, Feng-Qian;Cheng, Xiao-Xia;Wang, Shao

作者机构：

关键词： Reverse Transcription Loop-mediated isothermal Amplification (RT-LAMP);Novel Duck reovirus (NDRV);detection;sensitivity;rapidity;specificity

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Novel Duck Reovirus (NDRV) is the major causative agent of the Duck Necrotic Hemorrhagic Hepatitis (DNHH), it causes huge economic losses to the duck industry in China. The affected ducks (including Muscovy, Mule, Peking ducks and Sheldrake) manifestthe main clinical symptoms with hemorrhage and necrosis in the hepatitis which can cause high mortality in ducks. Develop a rapid and simple detection is the most efficient way for prevention and control the disease in short time. In this study, a Reverse Transcription Loop-mediated isothermal Amplification (RT-LAMP) assay was employed for detecting the disease. The sensitivity of RT-LAMP assay was 1000 folds greater and the detection limit was 2.8x10~(-3) ng RNA. In addition, the specificity results shown that it was no any cross-reaction with other duck's viruses. The RT-LAMP assay may be a good method for specific diagnosis of NDRV infection in fewer well-equipped laboratories, especially in farm or rural veterinary clinics.

分类号： S

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