Identification of a new gene encoding 5-enolpyruvylshikimate-3-phosphate synthase using genomic library construction strategy

文献类型： 外文期刊

第一作者： Zhou, Chang-Yan

作者： Zhou, Chang-Yan;Cai, Run;Wu, Ai-Zhong;Zhou, Chang-Yan;Tian, Yong-Sheng;Xu, Zhi-Sheng;Zhao, Wei;Chen, Chen;Bao, Wen-Hua;Bian, Lin;Wu, Ai-Zhong

作者机构：

关键词： EPSPS;Pseudomonas fluorescens;Enzyme kinetic values;Genomic library

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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年卷期：

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收录情况： SCI

摘要： Applying the genomic library construction strategy and colony screening, a new aroA gene encoding 5-enolpyruvylshikimate-3-phosphate synthase has been identified, cloned and overexpressed in Escherichia coli, and the enzyme was purified to homogeneity. Kinetic analysis of the AroA P.fluorescens indicated that the full-length enzyme exhibits 10-fold increased IC50 and an approximately 38-fold increased K i for glyphosate compared to those of the AroA E.coli , while retaining high affinity for the substrate phosphoenolpyruvate. Furthermore, we have transformed the new aroA P.fluorescens gene into Arabidopsis thaliana via a floral dip method, and demonstrated that transgenic A. thaliana plants exhibit significant glyphosate resistance when compared with the wild type.

分类号： Q7

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