Determining hydrogen cyanamide in fruit by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and HPLC with fluorescence detection

文献类型： 外文期刊

第一作者： Cai, Xiao-Ming

作者： Cai, Xiao-Ming;Zhao, Hua;Wu, Min;Hu, Xiu-Qing;He, Hong-Mei;Zhang, Chang-Peng;Zhang, Chun-Rong;Li, Zhen;Cai, Xiao-Ming;Zhao, Hua;Wu, Min;Hu, Xiu-Qing;He, Hong-Mei;Zhang, Chang-Peng;Zhang, Chun-Rong;Li, Zhen;Cai, Xiao-Ming;Zhao, Hua;Wu, Min;Hu, Xiu-Qing;He, Hong-Mei;Zhang, Chang-Peng;Zhang, Chun-Rong;Li, Zhen

作者机构：

关键词： 6-Aminoquinolyl- Nhydroxysuccinimidyl carbamate;Fluorescence detection;Fruit;HPLC;Hydrogen cyanamide;Liquid-liquid extraction

期刊名称：CHROMATOGRAPHIA （ 影响因子：2.044； 五年影响因子：1.725 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Hydrogen cyanamide is a plant-growth regulator used on fruit crops, and has a high toxicity. In this study, a simple and sensitive method for detecting hydrogen cyanamide in fruit was developed. In the proposed method, cyanamide was extracted with water from homogenized fruit samples (grape, kiwi, and peach). The extract was purified through twice liquid-liquid extraction. Then, the purified extract was derivatized with 6-aminoquinolyl- N-hydroxysuccinimidyl carbamate. Finally, the cyanamide derivative was analyzed using HPLC with fluorescence detection. This method was validated at 2, 0.2, and 0.01 mg kg ~(-1) cyanamide. The recovery rates were between 87.1 and 96.6 %, and the intraday and interday reproducibilities were within 1.8 and 2.2 %, respectively. The limits of detection and quantification were 2.4 and 8 μg kg ~(-1) cyanamide, respectively. This method was applied to explore the residue degradation of cyanamide in the field. The results show that the half-life of cyanamide residues on grapes was <2.0 days. Compared with the previous methods, this simple method is sensitive and can be applied for detecting cyanamide in a wide range of fruits.

分类号： O65

  • 相关文献

[1]Determination of tryptophan in bee pollen and royal jelly by high-performance liquid chromatography with fluorescence detection. Zhang, Jin-zhen,Xue, Xiao-feng,Zhou, Jin-hui,Chen, Fang,Wu, Li-ming,Li, Yi,Zhao, Jing. 2009

[2]Occurrence of patulin in various fruit products and dietary exposure assessment for consumers in China. Ji, Xiaofeng,Li, Rui,Yang, Hua,Qi, Peipei,Xiao, Yingping,Qian, Mingrong,Ji, Xiaofeng,Li, Rui,Yang, Hua,Qi, Peipei,Xiao, Yingping,Qian, Mingrong,Ji, Xiaofeng,Li, Rui,Yang, Hua,Qi, Peipei,Xiao, Yingping,Qian, Mingrong.

[3]Hydrogen cyanamide improves endodormancy release and blooming associated with endogenous hormones in "Summit' sweet cherry trees. Wang, Lei,Zhang, Caixi,Zhu, Lina,Yu, Xiuming,Li, Jiefa,Lou, Yusui,Xu, Wenping,Wang, Shiping,Ma, Chao,Huang, Jiancheng,Zhu, Lina.

[4]Determination of Water- and Methanol-Extractable Pentachlorophenol in Soils Using Vortex-assisted Liquid-Liquid Extraction and Gas Chromatography. Yu Xiong-Sheng,Liu Yong,Lou Jun,Feng Xiao-Li,Wang Hai-Zhen,Xu Jian-Ming,Liu Yong. 2015

[5]An ionic liquid-based nanofluid of titanium dioxide nanoparticles for effervescence-assisted dispersive liquid-liquid extraction for acaricide detection. Wu, Xiaoling,Li, Xin,Yang, Miyi,Zeng, Haozhe,Zhang, Sanbing,Lu, Runhua,Gao, Haixiang,Xu, Donghui,Xu, Donghui.

[6]Simultaneous determination of trace migration of phthalate esters in honey and royal jelly by GC-MS. Zhou, Jinhui,Diao, Qingyun,Li, Yi,Zhou, Jinhui,Li, Yi,Zhou, Jinhui,Li, Yi,Qi, Yitao,Wu, Hongmei,Tian, Feifei.

[7]Magnetic metal-organic framework MIL-100(Fe) microspheres for the magnetic solid-phase extraction of trace polycyclic aromatic hydrocarbons from water samples. Du, Fuyou,Qin, Qun,Ruan, Guihua,Li, Jianping,Yang, Xianqing,Li, Laihao,Du, Fuyou,Ruan, Guihua,Li, Jianping.

[8]Direct Fluorescent Detection of a Polymethoxyflavone in Cell Culture and Mouse Tissue. Chen, Jingjing,Song, Mingyue,Wu, Xian,Zheng, Jinkai,He, Lili,McClements, David Julian,Decker, Eric,Xiao, Hang,Xiao, Hang,Zheng, Jinkai.

[9]Graphene oxide: An adsorbent for the extraction and quantification of aflatoxins in peanuts by high-performance liquid chromatography. Yu, Li,Li, Peiwu,Zhang, Qi,Zhang, Wen,Ding, Xiaoxia,Wang, Xiupin,Yu, Li,Zhang, Qi,Zhang, Wen,Ding, Xiaoxia,Wang, Xiupin,Li, Peiwu,Li, Peiwu,Ding, Xiaoxia,Yu, Li,Li, Peiwu,Zhang, Wen,Wang, Xiupin.

[10]Ultrasensitive fluorescence detection of nucleic acids using exonuclease III-induced cascade two-stage isothermal amplification-mediated zinc (II)-protoporphyrin IX/G-quadruplex supramolecular fluorescent nanotags. Xue, Qingwang,Lv, Yanqin,Zhang, Yuanfu,Xu, Shulin,Li, Rui,Yue, Qiaoli,Li, Haibo,Wang, Lei,Liu, Jifeng,Gu, Xiaohong,Zhang, Shuqiu.

[11]LC Analysis of Aliphatic Primary Amines and Diamines After Derivatization with 2,6-Dimethyl-4-quinolinecarboxylic Acid N-Hydroxysuccinimide Ester. Huang, Ke-Jing,Wei, Cai-Yun,Xie, Wan-Zhen,Liu, Yan-Ming,Chen, Yong-Hong,Zhang, Jun-Feng.

[12]A DNA nanomachine based on rolling circle amplification-bridged two-stage exonuclease III-assisted recycling strategy for label-free multi-amplified biosensing of nucleic acid. Xue, Qingwang,Lv, Yanqin,Cui, Hui,Liu, Jifeng,Gu, Xiaohong,Zhang, Shuqiu,Liu, Jifeng.

[13]Microarray analysis of differentially expressed genes engaged in fruit development between Prunus mume and Prunus armeniaca. Li, Xiaoying,Korir, Nicholas Kibet,Shangguan, Lingfei,Han, Jian,Fang, Jinggui,Wang, Yuzhu,Liu, Lili,Chen, Ming. 2012

[14]Simultaneous Quantitation of Organic Acids and Monosaccharides by High-Performance Liquid Chromatography. Lu Xin,Song Guohui,Huang Jinian,Li Ruihong,Hagiwara, Shoji,Nabetani, Hiroshi. 2015

[15]Principles and Applications of Hyperspectral Imaging Technique in Quality and Safety Inspection of Fruits and Vegetables. Zhang Bao-hua,Zhang Bao-hua,Li Jiang-bo,Fan Shu-xiang,Huang Wen-qian,Zhang Chi,Wang Qing-yan,Xiao Guang-dong. 2014

[16]Effects of Citric Acid Treatments on the Postharvest Fruit Quality of Ziziphus jujuba Mill. 'Linyilizao'. Zhao, Z. H.,Liu, M. J.,Liu, P.,Liu, X. Y.,Dai, L.,Yang, L.,Tian, S. L.. 2009

[17]Variations in contents of (-)-epicatechin and activities of phenylalanine ammonialyase and polyphenol oxidase of longan fruit during development. Yu, Chunyan,Sun, Jian,Yang, Bao,Jiang, Yueming,Xiang, Xu. 2010

[18]GC-MS Analysis of Volatile Components in Chinese Wild Strawberry (F. nilgerrensis Schlecht.). Zhao, M. Z.,Wang, J.,Wang, Z. W.,Qian, Y. M.,Wu, W. M..

[19]Radiochromic film dosimetry for UV-C treatments of apple fruit. Yan, Ruixiang,Yun, Juan,Fan, Xuetong,Yun, Juan.

[20]Morphological, cytological and fertility consequences of a spontaneous tetraploid of the diploid pear (Pyrus pyrifolia Nakai) cultivar 'Cuiguan'. Wang, Xiaoqing,Shi, Chunhui,Zhang, Xueying,Duan, Ke,Luo, Jun,Wang, Xiaoqing,Shi, Chunhui,Zhang, Xueying,Duan, Ke,Luo, Jun,Wang, Haihai.

作者其他论文 更多>>

微信小程序