Parallel assembly for multiple site-directed mutagenesis of plasmids

文献类型： 外文期刊

第一作者： Yan, Pu

作者： Yan, Pu;Shen, Wentao;Zhou, Peng;Duan, Jun;Gao, XinZheng

作者机构：

关键词： Golden Gate cloning;Multiple mutagenesis;Parallel assembly;Plasmid

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A parallel assembly method for multiple site-directed mutagenesis of plasmids was developed here based on Golden Gate cloning. It takes advantage of type IIs restriction enzymes and T4 DNA ligase to assemble multiple DNA fragments into a plasmid by a defined order. This method can accommodate multiple plasmid mutagenesis at any desired position with all three sequence modification types (substitution, deletion, and insertion) simultaneously. Furthermore, it can be used to create otherwise difficult-to-make mutants - larger deletions and insertions and mutagenesis on larger plasmids. The processes of mutagenesis can be completed quickly by a single restriction-ligation reaction.

分类号： Q5

  • 相关文献

[1]Modular construction of plasmids by parallel assembly of linear vector components. Gao, XinZheng,Li, Yuenan,Yan, Pu,Shen, Wentao,Li, Xiaoying,Zhou, Peng.

[2]Fitness Advantage of mcr-1-Bearing IncI2 and IncX4 Plasmids in Vitro. Wu, Renjie,Yi, Ling-xian,Yu, Lin-feng,Wang, Jing,Liu, Yiyun,Chen, Xiaojie,Lv, Luchao,Yang, Jun,Liu, Jian-Hua,Chen, Xiaojie. 2018

[3]pirAB(vp)-Bearing Vibrio parahaemolyticus and Vibrio campbellii Pathogens Isolated from the Same AHPND-Affected Pond Possess Highly Similar Pathogenic Plasmids. Dong, Xuan,Wang, Hailiang,Zou, Peizhuo,Xie, Guosi,Wan, Xiaoyuan,Yang, Qian,Zhu, Yanping,Chen, Mengmeng,Guo, Chengcheng,Wang, Wenchao,Huang, Jie,Bi, Dexi,Zou, Peizhuo,Chen, Mengmeng,Liu, Zhen. 2017

[4]Multidrug resistance genes in staphylococci from animals that confer resistance to critically and highly important antimicrobial agents in human medicine. Wendlandt, Sarah,Kadlec, Kristina,Fessler, Andrea T.,Schwarz, Stefan,Shen, Jianzhong,Wang, Yang,Wu, Congming,Li, Beibei,Zhang, Wan-Jiang.

[5]A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Peng, Ri-He,Xiong, Ai-Sheng,Yao, Quan-Hong.

[6]GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A.. 2016

[7]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[8]The technology of large-scale pharmaceutical plasmid purification by cetyltrimethylammonium bromide and Tritonx-114. Hou, S. H.,Zhu, H. F.,Zhang, Q.,Yuan, W. F.,Sun, H. C.. 2010

[9]Nickel-resistant determinant from Leptospirillum ferriphilum. Tian, Jian,Wu, Ningfeng,Li, Jiang,Liu, Yajie,Guo, Jun,Yao, Bin,Fan, Yunliu.

[10]Multiplex event-specific qualitative polymerase chain reaction for detecting three transgenic rice lines and application of a standard plasmid as a quantitative reference molecule. Wang, Xiaofu,Shen, Wenbiao,Wang, Xiaofu,Chen, Xiaoyun,Xu, Junfeng,Wang, Pengfei.

[11]Inducible expression of calreticulin-N58 in Pichia pastoris by high density cell culture. Su, D. X.,Zhang, A. L.,Liu, Z. W.,Luo, J. X.,Zhang, T. Y.,Luo, J. X.,Zhang, T. Y.,Yi, G. H.,Rao, L. Y.,Zhou, Z. J..

作者其他论文 更多>>

微信小程序