Gene expression profiling of hybridoma cells after bursal-derived bioactive factor BP5 treatment

文献类型： 外文期刊

第一作者： Feng, Xiu L.

作者： Feng, Xiu L.;Cao, Rui B.;Zhou, Bin;Li, De Y.;Zhang, Yuan P.;Liu, Ke;Liu, Xiao D.;Qiu, Ya F.;Chen, Pu Y.;Liu, Qing T.;Wei, Jian C.;Qiu, Ya F.;Ma, Zhi Y.

作者机构：

关键词： Bursal-derived BP5;Gene microarray;Go analysis;p53 pathway;p53 expression

期刊名称：AMINO ACIDS （ 影响因子：3.52； 五年影响因子：3.6 ）

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收录情况： SCI

摘要： Bursa of Fabricius is the acknowledged vital humoral immune system for B cell differentiation and antibody production. To study the molecular mechanism underlying the effect of bursal-derived BP5, we used gene microarray to analyze the genomic expression profiling of BP5-treated hybridoma cells. BP5 exhibited an immunomodulatory effect on antibody production in hybridoma cells and induced alterations in the gene expression profiles related to the immune-related biological processes, such as T cell activation and proliferation, B cell activation, B cell-mediated immunity, and cytokines cytokine production involved in immune response. In addition, 26 biological pathways associated with immunomodulatory functions were regulated in BP5-treated hybridoma cells, in which p53 signal pathway played an important role in antitumor. Among these regulated genes, 12 differentially expressed genes were verified by qRT-PCR. The activation of p53 activity by BP5 was further confirmed by p53 luciferase reporter assay and p53 expression. Our data revealed that bursal-derived BP5 could regulate various immune-related cellular processes, including antitumor factor p53 signal pathway, perhaps partially accounting for the reported immunomodulatory roles and novel antiproliferation on tumor cells functions of bursal-derived bioactive factor BP5.

分类号： Q5

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