Loop-mediated isothermal amplification (LAMP) method based on two species-specific primer sets for the rapid identification of Chinese Babesia bovis and B. bigemina

文献类型： 外文期刊

第一作者： Liu, Aihong

作者： Liu, Aihong;Guan, Guiquan;Du, Pengfei;Gou, Huitian;Liu, Zhijie;Liu, Junlong;Ma, Milin;Yang, Jifei;Li, Youquan;Niu, Qinli;Ren, Qiaoyun;Bai, Qi;Yin, Hong;Luo, Jianxun

作者机构：

关键词： Babesia bovis;Babesia bigemina;LAMP;Detection;Differentiation

期刊名称：PARASITOLOGY INTERNATIONAL （ 影响因子：2.23； 五年影响因子：2.281 ）

ISSN：

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收录情况： SCI

摘要： Bovine babesiosis is a tick-transmitted hemoprotozoan disease that is mainly caused by Babesia bovis and/or Babesia bigemina and is characterized by significant morbidity and mortality worldwide. This disease is widespread in most parts of China. However, it is difficult to rapidly discriminate between the B. bovis and B. bigemina species. To detect and distinguish these species, a loop-mediated isothermal amplification (LAMP) platform that targets specific sequences of the internal transcribed spacer (ITS) genes was developed. Specificity testing revealed that there was no cross-reaction with the other tick-borne parasites B. ovate, B. major, unnamed bovine Babesia, Theileria annulata, Theileria sinensis, Theileria sergenti, and Anaplasma marginale, or with bovine white blood cells. The sensitivity of the LAMP method was 0.1 pg DNA for both B. bovis and B. bigemina, which was superior to that of the classical PCR methods. This assay was evaluated for its diagnostic utility using blood samples collected from experimentally and naturally infected cattle in China. These findings indicate that the Babesia species-specific LAMP assay may have potential clinical application in the detection and differentiation of Babesia species, particularly in countries in which babesiosis is endemic.

分类号： Q18

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