Expression of an Avian Influenza Virus (H5N1) Hemagglutinin Gene in Transgenic Lotus corniculatus
文献类型: 外文期刊
第一作者: Guo, Qian-Qian
作者: Guo, Qian-Qian;Zhang, Zhan-Lu;Jiang, San-Jie;Ma, Jiang-Tao;Wu, Yan-Min;Zhang, Zhan-Lu;Xue, Wen-Tong;Ma, Jiang-Tao
作者机构:
关键词: H5N1;Hemagglutinin;Lotus corniculatus;Matrix attachment;regions;Plant bioreactor
期刊名称:PLANT MOLECULAR BIOLOGY REPORTER ( 影响因子:1.595; 五年影响因子:2.042 )
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年卷期:
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收录情况: SCI
摘要: Cotyledons from 8-day-old seedlings of Lotus corniculatus were transformed with a gene encoding the avian influenza (H5N1) hemagglutinin (HA) under the control of the CaMV35S promoter along with matrix attachment regions using Agrobacterium-mediated transformation. The HA gene was detected in genomic DNA from transformed plants by PCR and Southern blotting. Immunoblot analysis of the plant-derived protein revealed the presence of the avian influenza (H5N1) HA. The ELISA results showed that the maximum expression level of the HA protein is up to 0.00786% of the total soluble protein. This is the first report of successful expression of the avian influenza (H5N1) HA in transgenic L. corniculatus, cultivar Leo.
分类号: Q94
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