NIa-pro of Papaya ringspot virus interacts with papaya methionine sulfoxide reductase B1

文献类型: 外文期刊

第一作者: Gao, Le

作者: Gao, Le;Shen, Wentao;Yan, Pu;Tuo, Decai;Li, Xiaoying;Zhou, Peng;Gao, Le;Shen, Wentao;Yan, Pu;Tuo, Decai;Li, Xiaoying;Zhou, Peng;Gao, Le

作者机构:

关键词: Interaction;Methionine sulfoxide reductase B1;NIa-Pro;Potyviridae;PRSV;ROS

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

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收录情况: SCI

摘要: A chloroplast-localized papaya methionine sulfoxide reductase B1 (PaMsrB1) interacting with Papaya ringspot virus (PRSV) NIa-Pro was identified using a Sos recruitment two-hybrid system (SRS). SRS analysis of several deletion mutants of PRSV NIa-Pro and PaMsrB1 demonstrated that the C-terminal (residues 133-239) fragment of PRSV NIa-Pro and residues 112-175 of PaMsrB1 were necessary for this interaction between PRSV NIa-Pro and PaMsrB1. MsrB1 can repair Met-oxidized proteins damaged by reactive oxygen species (ROS). We confirmed that PRSV infection leads to ROS accumulation and a slight upregulation of level PaMsrB1 mRNA in papaya. This interaction between PaMsrB1 with PRSV NIa-Pro may disturb the import of PaMsrB1 into the chloroplasts. These results suggest that this specific interaction could interfere with PaMsrB1 into the chloroplasts to scavenge ROS caused by PRSV infection. This may be a novel mechanism of PRSV towards the host defense.

分类号: R37

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