Tissue-specific transcriptome profiling of Plutella xylostella third instar larval midgut

文献类型： 外文期刊

第一作者： Xie, Wen

作者： Xie, Wen;Lei, Yanyuan;Fu, Wei;Yang, Zhongxia;Zhu, Xun;Guo, Zhaojiang;Wu, Qingjun;Wang, Shaoli;Xu, Baoyun;Zhang, Youjun;Zhou, Xuguo

作者机构：

关键词： Expressed sequence tag;Illumina sequencing;Insecticide re-sistance;Midgut;Plutella xylostella

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES （ 影响因子：6.58； 五年影响因子：6.478 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The larval midgut of diamondback moth, Plutella xylostella, is a dynamic tissue that interfaces with a diverse array of physiological and toxicological processes, including nutrient digestion and allocation, xenobiotic detoxification, innate and adaptive immune response, and pathogen defense. Despite its enormous agricultural importance, the genomic resources for P. xylostella are surprisingly scarce. In this study, a Bt resistant P. xylostella strain was subjected to the in-depth transcriptome analysis to identify genes and gene networks putatively involved in various physiological and toxicological processes in the P. xylostella larval midgut. Using Illumina deep sequencing, we obtained roughly 40 million reads containing approxi-mately 3.6 gigabases of sequence data. De novo assembly generated 63,312 ESTs with an av-erage read length of 416bp, and approximately half of the P. xylostella sequences (45.4%, 28,768) showed similarity to the non-redundant database in GenBank with a cut-off E-value below 10 -5. Among them, 11,092 unigenes were assigned to one or multiple GO terms and 16,732 unigenes were assigned to 226 specific pathways. In-depth analysis indentified genes putatively involved in insecticide resistance, nutrient digestion, and innate immune defense. Besides conventional detoxification enzymes and insecticide targets, novel genes, including 28 chymotrypsins and 53 ABC transporters, have been uncovered in the P. xylostella larval midgut transcriptome; which are potentially linked to the Bt toxicity and resistance. Furthermore, an unexpectedly high number of ESTs, including 46 serpins and 7 lysozymes, were predicted to be involved in the immune defense. As the first tissue-specific transcriptome analysis of P. xylostella, this study sheds light on the molecular understanding of insecticide resistance, especially Bt resistance in an agriculturally important insect pest, and lays the foundation for future functional genomics research. In addition, current sequencing effort greatly enriched the existing P. xylostella EST database, and makes RNAseq a viable option in the future genomic analysis.

分类号： Q

  • 相关文献

[1]Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage. Chen, Jine,Li, Fengbo,Liu, Yan,Shen, Weifeng,Du, Xin,He, Lihua,Meng, Zhiqi,Wang, Yongqiang,Ma, Xianliang. 2018

[2]Down-regulation of aminopeptidase N and ABC transporter subfamily G transcripts in Cry1Ab and Cry1Ac resistant Asian corn borer, Ostrinia furnacalis (Lepidoptera: Crambidae). Wang, Yueqin,Wang, Yidong,Bai, Shuxiong,Wang, Zhenying,He, Kanglai,Coates, Brad S.. 2017

[3]Activities of Digestive Enzymes in the Omnivorous Pest Apolygus lucorum (Hemiptera: Miridae). Yuan, Wei,Wu, Kongming,Zhao, Xincheng.

[4]The physiological differentiation along the midgut of Bombyx mori - inspirations from proteomics and gene expression patterns of the secreted proteins in the ectoperitrophic space. Qu, M.,Yang, J.,Yang, Q.,Liu, L.,Qu, M.,Yang, J.,Yang, Q.,Yang, Q.. 2018

[5]Reduction of Bacillus thuringiensis Cry1Ac toxicity against Helicoverpa armigera by a soluble toxin-binding cadherin fragment. Liu, Chenxi,Wu, Kongming,Gao, Yulin,Ning, Changming,Wu, Yidong,Ning, Changming,Oppert, Brenda.

[6]Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis. Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xu, Li,Yan, Wei,Li, Qian-Long,Xiao, Sheng-Yan,Fu, Xu-Liang,Wang, Wei,Li, Nan,Shen, Zhong-Yuan,Yue, Ya-Jie,Tang, Xu-Dong,Xiao, Sheng-Yan,Li, Nan,Shen, Zhong-Yuan.

[7]Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Deng, Xiangyuan.

[8]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[9]Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Li, Mu-Wang,Wang, Xiu-Ye,Liu, Ting,Qin, Guang-Xing,Guo, Xi-Jie,Wang, Xiu,Zhao, Pan,Guo, Xi-Jie. 2009

[10]Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan. 2012

[11]Digital Gene Expression analysis in the midgut of 4008 silkworm strain infected with cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie. 2014

[12]iTRAQ-based quantitative proteomic analysis of midgut in silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-Yuan,Shang, Meng-Ke,Qin, Guang-Xing,Hou, Cheng-Xiang,Guo, Xi-Jie.

[13]Analysis of midgut gene expression profiles from different silkworm varieties after exposure to high temperature. Li, Qing Rong,Xiao, Yang,Wu, Fu Quan,Ye, Ming Qiang,Luo, Guo Qing,Xing, Dong Xu,Li, Li,Yang, Qiong.

[14]Autophagy precedes apoptosis during the remodeling of silkworm larval midgut. Franzetti, Eleonora,Grimaldi, Annalisa,Tettamanti, Gianluca,Huang, Zhi-Jun,Shi, Yan-Xia,Xie, Kun,Deng, Xiao-Juan,Li, Jian-Ping,Li, Qing-Rong,Yang, Wan-Ying,Zeng, Wen-Nian,Cao, Yang,Li, Qing-Rong,Casartelli, Morena,Deng, Hui-Min,Feng, Qili,Cappellozza, Silvia,Xia, Qingyou.

[15]Molecular characterization of the genomic region harboring the BYDV-resistance gene Bdv2 in wheat. Xin, Z.,Zhang, Z.,Gao, L.,Ma, Q.,Liu, Y.. 2009

[16]Development and validation of 89 novel expressed sequence tag-derived microsatellite markers in blood clam, Tegillarca granosa. Teng, Shuangshuang,Fang, Jun,Cai, Yilong,Chai, Xueliang,Xiao, Guoqiang,Teng, Shuangshuang,Fang, Jun,Cai, Yilong,Chai, Xueliang,Xiao, Guoqiang,Teng, Shuangshuang,Fang, Jun,Cai, Yilong,Chai, Xueliang,Xiao, Guoqiang. 2017

[17]Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequencetags. Zhu, ZD,Huo, YL,Wang, XM,Huang, JB,Wu, XF. 2004

[18]Characterization of 28 polymorphic SSR loci from Meretrix lamarkii Deshayes. Liu, Bo,Teng, Shuang-shuang,Chai, Xue-Liang,Shao, Yan-Qing,Xiao, Guo-Qiang,Fang, Jun,Zhang, Jiong-ming,Liu, Bo,Teng, Shuang-shuang,Chai, Xue-Liang,Shao, Yan-Qing,Xiao, Guo-Qiang,Fang, Jun,Zhang, Jiong-ming,Liu, Bo. 2014

[19]In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene. Liu, Y. F.,Wan, H. J.,Wei, Y. P.,Wang, R. Q.,Ruan, M. Y.,Ye, Q. J.,Li, Z. M.,Zhou, G. Z.,Yao, Z. P.,Yang, Y. J.. 2015

[20]Development of EST-PCR markers for Thinopyrum intermedium chromosome 2Ai#2 and their application in characterization of novel wheat-grass recombinants. Wang, M. J.,Zhang, Y.,Lin, Z. S.,Ye, X. G.,Xin, Z. Y.,Wang, M. J.,Yuan, Y. P.,Zhang, Y.,Ma, W..

作者其他论文 更多>>

微信小程序