Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity

文献类型： 外文期刊

第一作者： Zhang, C.

作者： Zhang, C.;Song, Y.;Jiang, F.;Jiang, Y.;Zhu, C.;Wen, F.;Li, G.

作者机构：

关键词： Agrobacterium tumefaciens;Nicotiana tabacum;Oryza sativa;Potato virus Y;Rice stripe virus

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN：

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收录情况： SCI

摘要： To induce virus resistance in tobacco and rice we constructed hairpin RNA expression system harbouring inverted repeat fragments of coat protein cDNA of Potato virus Y (PVY) or Rice stripe virus (RSV). These structures were driven by three promoters [cauliflower mosaic virus 35S (CaMV 35S), polyubiqutin gene of maize (Ubi), and Pharbitis nil leucine zipper gene (PNZIP)] which have different tissue-specific activity. PVY resistance ratios were 65.18, 24.33 and 83.54 % in transgenic tobacco plants harboring p35S-PVY, pUbi-PVY and pPNZIP-PVY. RSV resistance was 16.21, 28.61 and 29.33 % in transgenic rice plants harboring p35S-RSV, pUbi-RSV and pPNZIP-RSV. Northern blotting and GUS assay demonstrated that virus resistance levels were related to promoter activity. Therefore, choice of the more effective and tissue-specific promoter to reinforce transcription of hpRNAs will favour the cultivation of highly virusresistant transgenic plants.

分类号： Q94

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