Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods

文献类型： 外文期刊

第一作者： Qiu, Jiying

作者： Qiu, Jiying;Chen, Leilei;Zhu, Qingjun;Wang, Wenliang;Sun, Xin;Liu, Xiaoyong;Du, Fangling;Wang, Daijie

作者机构：

关键词： antioxidants;peanut shell;DPPH radical;HPLC separation;TOF-MS

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

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年卷期：

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收录情况： SCI

摘要： Peanut shell, a byproduct in oil production, is rich in natural antioxidants. Here, a rapid and efficient method using DPPH-HPLC-DAD-TOF/MS was used for the first time to screen antioxidants in peanut shell. The method is based on the hypothesis that upon reaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas of compounds with potential antioxidant activities in the HPLC chromatogram will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS technique. With this method, three compounds possessing potential antioxidant activities were found abundantly in the methanolic extract of peanut shell. They were identified as 5,7-dihydroxychromone, eri-odictyol, and luteolin. The contents of these compounds were 0.59,0.92, and 2.36 mg/g, respectively, and luteolin possessed the strongest radical scavenging capacity. DPPH-HPLC-DAD-TOF/MS assay facilitated rapid identification and determination of natural antioxidants in peanut shell, which may be helpful for value-added utilization of peanut processing byproducts.

分类号： TS2

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