DEVELOPMENT OF A CHEMILUMINESCENT ELISA FOR DETERMINING OKADAIC ACID IN SHELLFISH

文献类型： 外文期刊

第一作者： Wang, Quan

作者： Wang, Quan;Liu, Ying-Chun;Jiang, Wei;Chen, Yong-Jun;Song, Ning-Ning;Li, Jian

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期刊名称：JOURNAL OF FOOD QUALITY （ 影响因子：2.45； 五年影响因子：2.679 ）

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收录情况： SCI

摘要： An indirect competitive enzyme-linked immunosorbent assay with chemilumines-cent (CL-ELISA) detection for okadaic acid (OA) in mussel muscle was developed. A hybridoma cell line secreting monoclonal antibody against OA was established after immunization of BALB/c mice with artificially synthesized OA-bovine serum albumin as antigen. Luminol solution was used as the substrate for horseradish peroxidase. The detection limit was 0.175 ng/g. The range of average fortified recovery was 91.8-102.6% when OAwas spiked in mussel muscle at levels of 50, 160 and 800 fig/kg. The standard curve for OA showed good linearity over the concentration range of 0.08125-20 ng/mL. The cross-reactivity with dinophysistoxinl and sax-itoxin was 51.82 and 0%, respectively.In a residue study, the results obtained by CL-ELISA correlated well within those obtained using the commercial ABRAXIS kit (ABRAXIS, Warminster, PA). The developed method is therefore suitable for detecting the residues of OA in shellfish.

分类号： TS2

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