Probing the molecular determinant of the lipase-specific foldase Lif26 for the interaction with its cognate Lip26

文献类型： 外文期刊

第一作者： Zheng, Xiaomei

作者： Zheng, Xiaomei;Tian, Jian;Wu, Ningfeng;Fan, Yunliu

作者机构：

关键词： Acinetobacter sp.;Lipase;Lipase specific foldase;Protein interaction;Site-directed mutagenesis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： As a steric chaperone, the lipase-specific foldase Lif26 from Acinetobacter sp. XMZ-26 is required for correct folding of the lipase Lip26 in in vivo co-expression and in vitro refolding systems. Lif26 interacts with Lip26 as determined by yeast two hybrid assays in vivo and GST pull-down experiments in vitro. To study the molecular determinants of the interaction between Lif26 and Lip26, a homology model-based screening of residues, molecular dynamics (MD) simulation-based calculation of interaction energies, and site-directed mutagenesis to alter individual screened residues were applied. One conserved amino acid in the C-terminal mini-domain of Lif26, Arg332, was involved in the interaction with Lip26. Arg332 could not be replaced by any other residue, based on saturated site-directed mutagenesis, and it formed a conserved and stable salt bridge with Glu112 of Lip26, which may contribute to binding specificity. The residues surrounding Arg332, such as Trp288 in α9, likely stabilized Arg332 in the proper conformation for the interaction with Lip26.

分类号： Q71

  • 相关文献

[1]Characterization of a novel lipase and its specific foldase from Acinetobacter sp XMZ-26. Zheng, Xiaomei,Wu, Ningfeng,Fan, Yunliu. 2012

[2]Cloning, expression of a peroxiredoxin gene from Acinetobacter sp SM04 and characterization of its recombinant protein for zearalenone detoxification. Yu, Yuanshan,Wu, Hui,Yu, Yuanshan,Wu, Hui,Tang, Yuqian,Qiu, Liping. 2012

[3]Study on the tolerance and adsorption of five heavy metal ions by a radiation-resistant Acinetobacter sp.. Zhu Jing,Xie Yu-Qing,Song Su-Qin,Zhang Zhi-Dong,Wang Wei,Gu Mei-Ying,Tang Qi-Yong. 2013

[4]A novel cold-adapted lipase from Acinetobacter sp XMZ-26: gene cloning and characterisation. Zheng, Xiaomei,Chu, Xiaoyu,Zhang, Wei,Wu, Ningfeng,Fan, Yunliu. 2011

[5]A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis. Liu, Pei,Xu, Zhao-Shi,Lu, Pan-Pan,Hu, Di,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi. 2013

[6]Screening of candidate proteins interacting with IE-2 of Bombyx mori nucleopolyhedrovirus. Wu, Yejun,Wu, Yu,Wu, Yan,Tang, Hui,Wu, Huiling,Wang, Wenbing,Zhang, Guozheng.

[7]Isolation and molecular characterization of the Triticum aestivum L. ethylene-responsive factor 1 (TaERF1) that increases multiple stress tolerance. Xu, Zhao-Shi,Xia, Lan-Qin,Chen, Ming,Cheng, Xian-Guo,Zhang, Rui-Yue,Li, Lian-Cheng,Zhao, Yun-Xiang,Lu, Yan,Ni, Zhi-Yong,Liu, Li,Qiu, Zhi-Gang,Ma, You-Zhi.

[8]Functional identification of apple MdJAZ2 in Arabidopsis with reduced JA-sensitivity and increased stress tolerance. An, Xiu-Hong,Li, En-Mao,Xu, Kai,Cheng, Cun-Gang,Hao, Yu-Jin.

[9]Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea. Zhang, Dong Ling,Chen, Jian,Wang, Zhi Yong,Yu, Da Hui,Fan, Sigang.

[10]IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice. Song, Xiaoguang,Lu, Zefu,Yu, Hong,Shao, Gaoneng,Xiong, Jinsong,Meng, Xiangbing,Jing, Yanhui,Liu, Guifu,Xiong, Guosheng,Duan, Jingbo,Wang, Yonghong,Li, Jiayang,Song, Xiaoguang,Lu, Zefu,Yu, Hong,Shao, Gaoneng,Xiong, Jinsong,Meng, Xiangbing,Jing, Yanhui,Liu, Guifu,Xiong, Guosheng,Duan, Jingbo,Wang, Yonghong,Li, Jiayang,Shao, Gaoneng,Li, Jiayang,Yao, Xue-Feng,Liu, Chun-Ming,Li, Hongqing,Lu, Zefu,Xiong, Jinsong,Xiong, Guosheng. 2017

[11]Thellungiella halophila ThPIP1 gene enhances the tolerance of the transgenic rice to salt stress. Qiang Xiao-jing,Yu Guo-hong,Jiang Lin-lin,Sun Lin-lin,Zhang Shu-hui,Li Wei,Cheng Xian-guo,Sun Lin-lin,Zhang Shu-hui. 2015

[12]Double repression of soluble starch synthase genes SSIIa and SSIIIa in rice (Oryza sativa L.) uncovers interactive effects on the physicochemical properties of starch. Zhang, Guoyu,Cheng, Zhijun,Zhang, Xin,Guo, Xiuping,Su, Ning,Mao, Long,Wan, Jianmin,Zhang, Guoyu,Cheng, Zhijun,Zhang, Xin,Guo, Xiuping,Su, Ning,Mao, Long,Wan, Jianmin,Jiang, Ling,Wan, Jianmin.

[13]The apple WD40 protein MdTTG1 interacts with bHLH but not MYB proteins to regulate anthocyanin accumulation. An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,Tian, Yi. 2012

[14]Screen of Receptor-like Kinase OsWAK50 Intracellular Interacting Proteins by Yeast Two-hybrid System. Sun Li-Jing,Sun Ying,Zhang Qian,Lu Tie-Gang. 2012

[15]PtFCA from precocious trifoliate orange is regulated by alternative splicing and affects flowering time and root development in transgenic Arabidopsis. Ai, Xiao-Yan,Zhang, Jin-Zhi,Liu, Tian-Jia,Hu, Chun-Gen,Ai, Xiao-Yan. 2016

[16]Chloroplast in Plant-Virus Interaction. Zhao, Jinping,Liu, Yule,Zhao, Jinping,Zhang, Xian,Hong, Yiguo. 2016

[17]Construction of gateway-compatible yeast two-hybrid vectors for high throughput analysis of protein interaction. Wang, Weixia,Zhu, Tingheng,Yang, Xiao,Wang, Kun,Cui, Zhifeng,Wong, Hann-lin. 2010

[18]Protein sHSP26 improves chloroplast performance under heat stress by interacting with specific chloroplast proteins in maize (Zea mays). Hu, Xiuli,Yang, Yanfang,Gong, Fangping,Zhang, Li,Wu, Liuji,Li, Chaohao,Wang, Wei,Zhang, Dayong.

[19]The soybean GmDi19-5 interacts with GmLEA3.1 and increases sensitivity of transgenic plants to abiotic stresses. Feng, Zhi-Juan,Cui, Xiao-Yu,Cui, Xi-Yan,Chen, Ming,Yang, Guang-Xiao,Ma, You-Zhi,He, Guang-Yuan,Xu, Zhao-Shi. 2015

[20]Using an Improved Site-Directed Mutagenesis for the Creation of Constructs to Analyse Gene Function in Fusarium oxysporum f. sp cubense. Li, C. Y.,Yi, G. J.,Chen, S.,Sun, Q. M.,Zuo, C. W.,Huang, B. Z.,Wei, Y. R.,Huang, Y. H.,Wu, Y. L.,Xu, L. B.,Hu, C. H.. 2011

作者其他论文 更多>>

微信小程序