Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining
文献类型: 外文期刊
第一作者: Xu, Dong
作者: Xu, Dong;Li, Feng;Fan, Xiao;Zhang, Xiaowen;Ye, Naihao;Mou, Shanli;Gao, Zhengquan;Liang, Chengwei;Li, Demao
作者机构:
关键词: Biodiesel;BODIPY 505/515;Lipid determination;Nitrogen limitation;Tetraselmis subcordiformis
期刊名称:BIORESOURCE TECHNOLOGY ( 影响因子:9.642; 五年影响因子:9.237 )
ISSN:
年卷期:
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收录情况: SCI
摘要: BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga Tetraselmis subcordiformis. A concentration of 0.28μgml~(-1) and staining for 6min was optimal. Lipid bodies stained with BODIPY505/515 had a characteristic green fluorescence. Their volumes were determined using the sphere volume formula. Lipid accumulation under different nitrogen concentrations was analyzed. With an increase in NaNO_3 concentration from 0 to 240mg L~(-1), the maximum algal concentration increased from 8.23±0.62 (×10~5 cells ml~(-1)) to 1.61±0.13 (×10~6 cellsml~(-1)), while the maximum volume of intracellular neutral lipid decreased from 9.78±1.77 μm~3cell~(-1) to 6.00±0.59μm~3cell~(-1). A comparison of the lipid contents measured by BODIPY 505/515 staining and the gravimetric method showed a positive correlation coefficient of R~2=0.93. BODIPY 505/515 staining is a promising method in lipid quantitation in T. subcordiformis.
分类号: Q
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