Detection of virulence-associated genes in Staphylococcus aureus isolated from bovine clinical mastitis milk samples in Guangxi

文献类型: 外文期刊

第一作者: Yang, Feng Li

作者: Yang, Feng Li;Li, Xiao Shan;Liang, Xian Wei;Zhang, Xiu Fang;Qin, Guang Sheng;Yang, Bing Zhuang

作者机构:

关键词: Bovine mastitis;Staphylococcus aureus;Virulence-associated genes;Polymerase chain reaction (PCR)

期刊名称:TROPICAL ANIMAL HEALTH AND PRODUCTION ( 影响因子:1.559; 五年影响因子:1.681 )

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年卷期:

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收录情况: SCI

摘要: Staphylococcus aureus is recognized worldwide as a pathogen causing many serious diseases in humans and animals and is one of the most common etiological agents of clinical and subclinical bovine mastitis. The purpose of this study was to determine the presence of genes encoding clfA, fnbA, fnbB, cap5, cap8, hla, hlb, nuc, sea, and tst of S. aureus strains (n=39) isolated from bovine clinical mastitis in Guangxi by polymerase chain reaction amplification. The results of the present study indicated that all isolates were found to contain one or more virulence-associated genes. The most frequently encountered genes were fnbA (97%) and nuc (90%), followed by hla (85%) and hlb (82%), respectively. None of the investigated S. aureus strains harbored fnbB and sea genes. The data in the present study showed a relatively wide distribution of the genes fnbA and nuc among the investigated isolates, indicating that they play an important role on bovine mastitis pathogenesis. The study provides a valuable insight into the virulence-associated genes of this important pathogen.Digital Object Identifier http://dx.doi.org/10.1007/s11250-012-0143-z

分类号: S8

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