Lipase Immobilization on Hyper-Cross-Linked Polymer-Coated Silica for Biocatalytic Synthesis of Phytosterol Esters with Controllable Fatty Acid Composition

文献类型： 外文期刊

第一作者： Zheng, Ming-Ming

作者： Zheng, Ming-Ming;Huang, Feng-Hong;Wang, Lian;Guo, Ping-Mei;Deng, Qian-Chun;Zheng, Ming-Ming;Huang, Feng-Hong;Wang, Lian;Guo, Ping-Mei;Deng, Qian-Chun;Lu, Yong;Feng, Yu-Qi

作者机构：

关键词： phytosterol ester;biocatalysis;esterification;hyper-cross-linked polymer;fatty acid composition

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： In this study, a novel mixed-mode composite material, SiO2@P(MAA-co-VBC-co-DVB), was prepared via the hyper-cross-linking of its precursor, which was produced via suspension polymerization in the presence of SiO2 particles. Candida rugosa lipase (CRL) was immobilized on the SiO2@P(MAA-co-VBC-co-DVB) particles via hydrophobic and weak cation-exchange interaction. The resulting immobilized CRL showed much better thermal stability and reusability in comparison to free CRL. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different fatty acids to produce the corresponding phytosterol esters was developed. Six phytosterol esters with conversions above 92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 μmol/mL phytosterols, 160 μmol/mL linolenic acid, and 15 mg/mL CRL@HPCS at 300 rpm and 50 °C for 7 h in 30 mL of isooctane. The prepared phytosterol esters possessed a low acid value (≤0.86 mg of KOH/g), peroxide value (≤3.3 mequiv/kg), and conjugated diene value (≤1.74 mmol/kg) and high purity (≥97.8%) and fatty solubility (≥28.9 g/100 mL). All the characteristics favored the wide application of phytosterol esters with controllable fatty acid composition in different fields of functional food.

分类号： R15

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