Purification and characterization of a xylanase from Bacillus subtilis isolated from the degumming line

文献类型： 外文期刊

第一作者： Guo, Gang

作者： Guo, Gang;Liu, Zhengchu;Xu, Junfei;Feng, Xiangyuan;Duan, Shengwen;Zheng, Ke;Cheng, Lifeng;Liu, Jianping;Dai, Xiaoyang;Xie, Daping;Peng, Keqing

作者机构：

关键词： Bacillus subtilis;Degumming;Purification;Xylanase

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Xylanases catalyze the hydrolysis of xylan, a major hemicellulose component of cell wall besides cellulose in most plant species. To extract cellulose fibers, it will be invaluable to screen for more effective xylanase-producing microorganisms. In this paper a new strategy for easy screening of xylanase-producing strains from the degumming line was presented. Using this strategy, a weak-acidic, cellulase-free xylanase from Bacillus subtilis has been isolated, purified and characterized. The xylanase showed high specific activity (36,633.4 U/mg), presented stable characteristics and can be separated and purified simply, with molecular weight 23.3 kD, pI 9.63. It reached its optimal activity at pH 5.8 and 60 ??C, and retained over 80% of its maximal activity after pre-incubation at temperature 60 ??C or pH 4.6 ?? 6.4. Also, a two-step purification procedure based on the combination of ultrafiltration and gel filtration chromatography was introduced and described, achieving 17-fold purification with 68.11% yield. ? 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

分类号： R37

  • 相关文献

[1]Production of xylobiose from the autohydrolysis explosion liquor of corncob using Thermotoga maritima xylanase B (XynB) immobilized on nickel-chelated Eupergit C. Tan, S. S.,Li, D. Y.,Jiang, Z. Q.,Zhu, Y. P.,Shi, B.,Li, L. T..

[2]A new acidophilic endo-beta-1,4-xylanase from Penicillium oxalicum: cloning, purification, and insights into the influence of metal ions on xylanase activity. Liao, Hanpeng,Sun, Shaowei,Wang, Pan,Bi, Wenli,Wei, Zhong,Mei, Xinlan,Liu, Dongyang,Raza, Waseem,Shen, Qirong,Xu, Yangchun,Tan, Shiyong,Tan, Shiyong.

[3]Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp S9. Li, Ning,Meng, Kun,Wang, Yaru,Shi, Pengjun,Luo, Huiying,Bai, Yingguo,Yang, Peilong,Yao, Bin.

[4]Variation of xylanosomal subunit composition of Streptomyces olivaceoviridis by nitrogen sources. Deng, W,Jiang, ZQ,Li, LT,Wei, Y,Shi, B,Kusakabe, I.

[5]Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916. Chen, Zhiyi,Ng, T. B.,Zhang, Jie,Zhou, Mingguo,Song, Fuping,Lu, Fan,Liu, Youzhou. 2007

[6]Expression and Characterization of the Dictyoglomus thermophilum Rt46B.1 Xylanase Gene (xynB) in Bacillus subtilis. Zhang, Wei,Li, Guan,Zhang, Wei,Lou, Kai.

[7]The cellulose protection agent used in the oxidation degumming of ramie. Meng, Chaoran,Liu, Fengming,Li, Zhaoling,Yu, Chongwen,Yu, Chongwen.

[8]Study on the Impact of Degummed on Pineapple Leaf Fiber's Structures and Formaldehyde Adsorptions. Li, Ming-fu,He, Jun-yan,Zhang, Jin,Deng, Gan-ran,Lian, Wen-wei,Huang, Tao,Zhuang, Zhi-kai,Ou, Zhong-qing. 2014

[9]Study on the Fabrication of Degummed Tilapia Fish Scale and its Adsorption of Carmine. Zhu, Kunkun,Li, Bin,Zhu, Kunkun,Gong, Xiao,Luo, Yongyue,Yang, Changjin,Peng, Zheng,Peng, Xinting. 2014

[10]Effect of Steam Explosion on Degumming Efficiency and Physicochemical Characteristics of Banana Fiber. Sheng, Zhanwu,Gao, Jinhe,Jin, Zhiqiang,Zheng, Lili,Wang, Bizun,Sheng, Zhanwu,Jin, Zhiqiang,Dai, Haofu. 2014

[11]Symbiotic Streptomyces sp. TN119 GH 11 xylanase: a new pH-stable, protease- and SDS-resistant xylanase. Zhou, Junpei,Shi, Pengjun,Zhang, Rui,Huang, Huoqing,Meng, Kun,Yang, Peilong,Yao, Bin. 2011

[12]Isolation and Identification of a Newly Isolated Alternaria sp ND-16 and Characterization of Xylanase. Li, Yin,Liu, Zhiqiang,Liu, Zhiqiang,Li, Geng,Cui, Fengjie,Ping, Lifeng,Qiu, Chongyan,Yan, Lijiao,Yan, Lijiao. 2009

[13]An acid and highly thermostable xylanase from Phialophora sp G5. Zhang, Fan,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Yuan, Tiezheng,Huang, Huoqing,Yang, Peilong,Yao, Bin,Zhang, Fan,Miao, Lihong. 2011

[14]Cloning, Expression, and Characterization of a New Streptomyces sp S27 Xylanase for Which Xylobiose is the Main Hydrolysis Product. Li, Ning,Shi, Pengjun,Yang, Peilong,Wang, Yaru,Luo, Huiying,Bai, Yingguo,Zhou, Zhigang,Yao, Bin. 2009

[15]Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry. Zhao, Liang,Meng, Kun,Bai, Yingguo,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Wang, Yaru,Yang, Peilong,Yao, Bin,Zhao, Liang,Song, Wei. 2013

[16]Cloning of a New Xylanase Gene from Streptomyces sp TN119 Using a Modified Thermal Asymmetric Interlaced-PCR Specific for GC-Rich Genes and Biochemical Characterization. Zhou, Junpei,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Bai, Yingguo,Yao, Bin. 2010

[17]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

[18]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[19]The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Wang, Guozeng,Wu, Jingjing,Lin, Juan,Ye, Xiuyun,Yao, Bin.

[20]A novel, alkali-tolerant thermostable xylanase from Saccharomonospora viridis: direct gene cloning, expression and enzyme characterization. Wang, Ziyuan,Jin, Yi,Wu, Huijun,Xie, Xiangming,Tian, Zhaofeng,Wu, Yuying.

作者其他论文 更多>>

微信小程序