Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein

文献类型: 外文期刊

第一作者: Han, Zongxi

作者: Han, Zongxi;Zhao, Fei;Shao, Yuhao;Liu, Xiaoli;Kong, Xiangang;Song, Yang;Liu, Shengwang

作者机构:

关键词: Avian infectious;Bronchitis;Coronavirus;Epitope;Monoclonal antibody;Nucleocapsid protein;Phylogenetic analysis

期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The nucleocapsid (N) protein of the infectious bronchitis virus (IBV) may play an essential role in the replication and translation of viral RNA. The N protein can also induce high titers of cross-reactive antibodies and cell-mediated immunity, which protects chickens from acute infection. In this study, we generated two monoclonal antibodies (mAbs), designated as 6D10 and 4F10, which were directed against the N protein of IBV using the whole viral particles as immunogens. Both of the mAbs do not cross react with Newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV) and subtype H9 avian influenza virus (AIV). After screening a phage display peptide library and peptide scanning, we identified two linear B-cell epitopes that were recognized by the mAbs 6D10 and 4F10, which corresponded to the amino acid sequences 242FGPRTK247 and 195DLIARAAKI203, respectively, in the IBV N protein. Alignments of amino acid sequences from a large number of IBV isolates indicated that the two epitopes, especially 242FGPRTK247, were well conserved among IBV strains. This conclusion was further confirmed by the relationships of 18 heterologous sequences to the 2 mAbs. The novel mAbs and the epitopes identified will be useful for developing diagnostic assays for IBV infections. ? 2012 Elsevier B.V.

分类号: R37

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