Porcine reproductive and respiratory syndrome virus ORF5a protein is essential for virus viability

文献类型： 外文期刊

第一作者： Lin, Tao

作者： Lin, Tao;Huang, Ting;Yao, Huochun;Fan, Hongjie;Sun, Lichang;Li, Yanhua;Liu, Runxia;Gao, Fei;Tong, Guangzhi;Wei, Zuzhang;Yuan, Shishan;Li, Yanhua;Wang, Xiaomin

作者机构：

关键词： Codon optimization;ORF5a;Porcine reproductive and respiratory syndrome virus;Viability

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： It has been shown that ORF5a protein in EAV is important but not essential for virus infectivity. In this study, we found that RNA changes in the overlapping region (1-104 nucleotide, nt) between ORF5 and ORF5a introduced by codon-optimized GP5 was lethal for virus viability, suggesting that the nt changes or amino acid (aa) mutations in the GP5 or ORF5a protein did not permit the production of infectious virus. Furthermore, inactivation of ORF5a expression in the context of type 1 (pSHE) and type 2 (pAJXM and pAPRRS) full-length PRRSV cDNA clones was lethal for the production of infectious virus, while viable PRRSV could be recovered by expressing ORF5a protein in trans, suggesting that ORF5a protein was essential for virus viability. Finally, ORF5a protein could be putatively extended to 63 aas by inactivation of the downstream stop codon candidates, thereby demonstrating that the C-terminus of ORF5a may be variable. ? 2012 Elsevier B.V.

分类号： R37

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