Purification and Characterization of Chromium-Binding Substances from High-Chromium Yeast

文献类型： 外文期刊

第一作者： Liu, Lu

作者： Liu, Lu;Lv, Jia-ping;Liu, Lu;Lv, Jia-ping;Uluko, Hankie

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关键词： high-chromium yeast;low-molecular-weight chromium-binding substance;high-molecular-weight chromium-binding substance;glucose tolerance factor

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： As a highly efficient and easily absorbable source of chromium, the identities of the chromium-binding substances in yeast remain unclear. In this study, a mild extraction procedure involving extraction with ammonia, three-gel filtration, and high-performance liquid chromatography was adopted to obtain two chromium-binding substances from high-chromium yeast. A low-molecular-weight chromium-binding substance was identified, with mass-to-charge ratios (m/z) of 769 and 712, which included glutamic acid, glycine, and cysteine in an approximate ratio of 1:1:1, as well as nicotinic acid and chromium(III). Furthermore, it significantly potentiated (by 51%) the action of insulin to stimulate the conversion of ~(14)C-glucose into lipid in adipocytes. A novel high-molecular-weight chromium-binding substance was also isolated: electrospray ionization tandem mass spectrometry tentatively identified it as HUB1 target protein-1, glyceraldehyde-3-phosphate dehydrogenase, or ribosomal protein L2A(L5A)(rp8)(YL6). This is the first report of a high-molecular-weight chromium-binding substance in yeast and merits further studies.

分类号： R15

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[1]Oral Administration of the High-Chromium Yeast Improve Blood Plasma Variables and Pancreatic Islet Tissue in Diabetic Mice. Liu, Lu,Lv, Jia-Ping,Jin, Wen. 2010

[2]Selection of high organic chromium yielding yeast strain after space piggyback experiment and its characteristic. Lv Jia-ping,Gao Yan-hong,Liu Lu,Gao Yan-hong. 2012

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