Identification and characterization of a gene encoding a putative lysophosphatidyl acyltransferase from Arachis hypogaea

文献类型： 外文期刊

第一作者： Chen, Si-Long

作者： Chen, Si-Long;Huang, Jia-Quan;Lei, Yong;Zhang, Yue-Ting;Ren, Xiao-Ping;Chen, Yu-Ning;Jiang, Hui-Fang;Yan, Li-Ying;Liao, Bo-Shou;Chen, Si-Long;Li, Yu-Rong;Chen, Si-Long

作者机构：

关键词： Arachis hypogaea;expression profile;gene cloning;lysophosphatidyl acyltransferase;structure and function

期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）

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收录情况： SCI

摘要： Lysophosphatidyl acyltransferase (LPAT) is the important enzyme responsible for the acylation of lysophosphatidic acid (LPA), leading to the generation of phosphatidic acid (PA) in plant. Its encoding gene is an essential candidate for oil crops to improve oil composition and increase seed oil content through genetic engineering. In this study, a full-length AhLPAT4 gene was isolated via cDNA library screening and rapid amplification of cDNA ends (RACE); our data demonstrated that AhLPAT4 had 1631 nucleotides, encoding a putative 43.8 kDa protein with 383 amino acid residues. The deduced protein included a conserved acyltransferase domain and four motifs (I-IV) with putative LPA and acyl-CoA catalytic and binding sites. Bioinformatic analysis indicated that AhLPAT4 contained four transmembrane domains (TMDs), localized to the endoplasmic reticulum (ER) membrane; detailed analysis indicated that motif I and motifs II—III in AhLPAT4 were separated by the third TMD, which located on cytosolic and ERluminal side respectively, and hydrophobic residues on the surface of AhLPAT4 protein fold to form a hydrophobic tunnel to accommodate the acyl chain. Subcellular localization analysis confirmed that AhLPAT4 was a cytoplasm protein. Phylogenetic analysisrevealed that AhLPAT4 had a high homology (63.7-78.3%) with putative LPAT4 proteins from Glycine max, Arabidopsis thaliana and Ricinus communis. AhLPAT4 was ubiquitously expressed in diverse tissues except in flower, which is almost undetectable. The expression analysis in different developmental stages in peanut seeds indicated that AhLPAT4 did not coincide with oil accumulation.

分类号： Q

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