Horizontal gene transfer does not occur between sFat-1 transgenic pigs and nontransgenic pigs

文献类型: 外文期刊

第一作者: Tang, M. X.

作者: Tang, M. X.;Qian, L. L.;Jiang, S. W.;Cui, W. T.;Li, K.;Tang, M. X.;Qian, L. L.;Jiang, S. W.;Cui, W. T.;Li, K.;Zheng, X. M.;Hou, J.

作者机构:

关键词: sFat-1 gene;Transgenic pig;Horizontal gene transfer;Suckling;Mating

期刊名称:THERIOGENOLOGY ( 影响因子:2.74; 五年影响因子:2.93 )

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收录情况: SCI

摘要: We previously generated and characterized synthesized fatty acid desaturase-1 (sFat-1) transgenic pigs that had increased concentrations of omega-3 unsaturated fatty acid in their meat. The objective was to assess whether the inserted foreign gene in sFat-1 transgenic pigs was able to transfer and integrate into the genome of nontransgenic pigs by suckling or mating. Tests for suckling-mediated horizontal gene transfer (HGT) included sFat-1 transgenic sows nursing nontransgenic piglets and sFat-1 transgenic piglets suckling nontransgenic sows. Tests for mating-mediated HGT were performed by male sFat-1 transgenic pigs mated with nontransgenic females and female sFat-1 transgenic pigs mated with nontransgenic males. Polymerase chain reaction was used to detect the sFat-1 gene fragment in various tissues sampled from nontransgenic pigs. The foreign target gene sFat-1 was not detected in the genomic DNA of various tissues and organs sampled from nontransgenic pigs. Therefore, we concluded that HGT from transgenic pigs to wild type pigs via suckling or mating was unlikely. (C) 2013 Elsevier Inc. All rights reserved.

分类号: Q95

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