Horizontal gene transfer does not occur between sFat-1 transgenic pigs and nontransgenic pigs
文献类型: 外文期刊
第一作者: Tang, M. X.
作者: Tang, M. X.;Qian, L. L.;Jiang, S. W.;Cui, W. T.;Li, K.;Tang, M. X.;Qian, L. L.;Jiang, S. W.;Cui, W. T.;Li, K.;Zheng, X. M.;Hou, J.
作者机构:
关键词: sFat-1 gene;Transgenic pig;Horizontal gene transfer;Suckling;Mating
期刊名称:THERIOGENOLOGY ( 影响因子:2.74; 五年影响因子:2.93 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: We previously generated and characterized synthesized fatty acid desaturase-1 (sFat-1) transgenic pigs that had increased concentrations of omega-3 unsaturated fatty acid in their meat. The objective was to assess whether the inserted foreign gene in sFat-1 transgenic pigs was able to transfer and integrate into the genome of nontransgenic pigs by suckling or mating. Tests for suckling-mediated horizontal gene transfer (HGT) included sFat-1 transgenic sows nursing nontransgenic piglets and sFat-1 transgenic piglets suckling nontransgenic sows. Tests for mating-mediated HGT were performed by male sFat-1 transgenic pigs mated with nontransgenic females and female sFat-1 transgenic pigs mated with nontransgenic males. Polymerase chain reaction was used to detect the sFat-1 gene fragment in various tissues sampled from nontransgenic pigs. The foreign target gene sFat-1 was not detected in the genomic DNA of various tissues and organs sampled from nontransgenic pigs. Therefore, we concluded that HGT from transgenic pigs to wild type pigs via suckling or mating was unlikely. (C) 2013 Elsevier Inc. All rights reserved.
分类号: Q95
- 相关文献
作者其他论文 更多>>
-
MGF360-12L of ASFV-SY18 is an immune-evasion protein that inhibits host type I IFN, NF-?B, and JAK/STAT
作者:Chen, Q.;Jiang, S. W.;Huang, S. Y.;Liang, Y.;Wang, X. X.;Jia, H.;Zhu, H. F.;Gao, X. T.
关键词:African swine fever virus; MGF360-12L; type I IFN; NF-?B; JAK; STAT
-
Effects of a 10-year irrigation with saline water on soil physico-chemical properties and cotton production
作者:Zhang, A.;Sun, C.;Zhang, J.;Li, K.;Dang, H.;Sun, J.;Wang, G.;Rahma, A. E.;Feng, D.
关键词:cotton yield; fiber quality; saline water irrigation; soil quality
-
Cold Temperature Regulation of Zoospore Release in Phytophthora sojae: The Genes That Differentially Expressed by Cold Temperature (vol 54, pg 618, 2018)
作者:Wang, Y. H.;Liu, T.;Hou, J.;Jin, X.;Rui, H.;Liu, T.
关键词:
-
Effects of chestnut tannins on intestinal morphology, barrier function, pro-inflammatory cytokine expression, microflora and antioxidant capacity in heat-stressed broilers
作者:Liu, H. W.;Zhao, J. S.;Li, K.;Deng, W.
关键词:barrier function; broiler; chestnut tannins; heat stress; intestinal microflora
-
Long non-coding MEG3 is a marker for skeletal muscle development and meat production traits in pigs
作者:Yu, X.;Sun, H.;Yang, Y.;Li, K.;Tang, Z.;Yu, X.;Tang, Z.;Wang, Z.
关键词:lncRNA; maternally expressed gene 3; swine; association analysis; muscle growth
-
Soil insect crop pests and their integrated management in East Africa: A review
作者:Li, K.;Rutikanga, A.;Zhang, S.;Yin, J.;Ya-zhong, C.;Zhang, X. X.;Rutikanga, A.;Rukazambuga, D. N. T.;Nyamwasa, I.;Sun, X.
关键词:Soil insect pests;Integrated pest management (IPM);Insecticides;East Africa
-
Cold Temperature Regulation of Zoospore Release in Phytophthora sojae: The Genes That Differentially Expressed by Cold Temperature
作者:Wang, Ya.;Liu, T.;Hou, J.;Jin, X.;Rui, H.;Liu, T.
关键词:cold temperature; Phytophthora sojae; zoospore release; differential gene expression