Tissue Distribution and Relative Quantitation of Experimental Infection with Peste Des Petits Ruminant Virus in Goats Using Real-Time PCR (TaqMan (R))

文献类型： 外文期刊

第一作者： Meng, Xuelian

作者： Meng, Xuelian;Dou, Yongxi;Zhai, Junjun;Shi, Xiaoni;Yan, Fengchao;Zhang, Hairui;Luo, Xuenong;Cai, Xuepeng

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期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

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收录情况： SCI

摘要： Peste des Petits Ruminants (PPR) is an acute, highly contagious and economically important viral disease of small ruminants, especially goats and sheep. Given the contagious nature of the disease, speed of diagnosis is essential in order to quarantine infected animals and initiate appropriate prophylactic measures. Here researchers reported the distribution of PPRV RNA in various tissues of goats experimentally infected subcutaneously with a vaccine virus (Nigeria 75/1) strain in relation to the time post inoculation (pi) by real-time PCR (rt RT-PCR) based on TaqMan (R) and was quantified as a fold change (2(-Delta Delta Ct) values) compared with that of blood at 3 dpi. PPRV levels at 3, 5 and 28 dpi were higher than those at the other time points, especially at 5 dpi. The PPRV level in thymus, tonsil, hilar lymph node and turbinate bone was higher than that of other tissues. The result suggested that the distribution of PPRV in goats were similar to that of viral antigens and rtRT-PCR was useful for the detection of PPRV in field samples. The higher levels of RNA detected at 28 dpi may suggest some level of persistent infection. Furthermore, it was shown that blood sample was the most valuable diagnostic material for use in an epidemic situation.

分类号： S

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