Expression patterns and binding properties of three pheromone binding proteins in the diamondback moth, Plutella xyllotella

文献类型： 外文期刊

第一作者： Liu, Yang

作者： Liu, Yang;Wang, Guirong

作者机构：

关键词： Plutella xyllotella;Pheromone binding protein;Prokaryotic expression;Fluorescence binding assay;Electroantennogram

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.354； 五年影响因子：3.045 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Pheromone binding proteins (PBPs) play a key role in transporting hydrophobic sex pheromone components emitted by con-specific female across aqueous sensillar lymph to the surface of olfactory receptor neurons. A number of PBPs have been cloned, however, details of their function are still largely unknown. Here three pheromone binding protein genes in the diamondback moth, Plutella xyllotella were cloned. The three PxylPBP genes are not only expressed in chemosensory tissues but also expressed in female reproductive organs and male legs. To better understand the functions of PxylPBPs in the initial steps of pheromone recognition, three PxylPBPs were expressed in Escherichia coli and the ligand-binding specificities of purified recombinant PBPs were investigated. Fluorescence binding assays indicate that three PxylPBPs not only robustly bound all four sex pheromone components but also significantly bound pheromone analogs with at least one double bond, while weakly bound tested plant volatiles. Although pheromone analogs bound PBPs, they could not elicit the moth's electrophysiological response. These experiments provide evidence that PxylPBPs have limited selectivity of pheromone components and analogs and some downstream components such as odor receptors might be involved in selectivity and specificity of pheromone perception in P. xyllotella

分类号： Q965

  • 相关文献

[1]Binding affinity of five PBPs to Ostrinia sex pheromones. Sun, Yaqi,He, Kanglai,Wang, Zhenying,Sun, Yaqi,Wanner, Kevin W.,Coates, Brad S.. 2017

[2]The effect of polyamines on the expression of olfactory related genes in the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae). Zhang, Zhichun,Luo, Guanghua,Zhang, Gufeng,Fang, Jichao,Wang, Manqun.

[3]Three odorant binding proteins may regulate the behavioural response of Chrysopa pallens to plant volatiles and the aphid alarm pheromone (E)-beta-farnesene. Z.-Q. Li,Chen, Z. -M.,S. Zhang,X.-M. Cai,J.-Y. Luo,S.-L. Dong,J.-J. Cui,Z.-M. Chen.

[4]Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Jing Zhu,Jin-Jie Cui,Shuang-Lin Dong.

[5]Odorant-binding proteins display high affinities for behavioral attractants and repellents in the natural predator Chrysopa pallens. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Si-BaoWang,Shuang-Lin Dong,Jin-Jie Cui.

[6]Two Minus-C odorant binding proteins from Helicoverpa armigera display higher ligand binding affinity at acidic pH than neutral pH. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Jin-Jie Cui,Yan Mab,Shuang-Lin Dong. 2013

[7]Identification and characterisation of two general odourant-binding proteins from the litchi fruit borer, Conopomorpha sinensis Bradley. Yao, Qiong,Xu, Shu,Dong, Yizhi,Lu, Kai,Chen, Bingxu.

[8]Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hubner). Zhang Tian-tao,Gu Shao-hua,Wu Kong-ming,Zhang Yong-jun,Guo Yu-yuan,Zhang Tian-tao,Guo Yu-yuan,Wang Wei-xuan,Zhang Zi-ding. 2012

[9]Electrophysiological and Behavioral Activity of Compounds in Metathoracic Glands of Adults of Tessaratoma Papillosa (Hemiptera: Pentatomidae). Wang, Yujie,Zhao, Dongxiang,Gao, Jinglin,Peng, Zhengqiang,Wang, Yujie,Li, Xiaona,Wang, Yun. 2011

[10]Composition of the volatiles from intact and mechanically pierced tea aphid-tea shoot complexes and their attraction to natural enemies of the tea aphid. Han, BY,Chen, ZM.

[11]Attraction. of winged tea aphids to tea plant volatiles measured by olfactometry and. electro-antennography. Han, B. Y.,Zhou, C. S.,Cui, L.. 2005

[12]Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals. Zhu, Xiao-Qiang,Gu, Shao-Hua,Cui, Huan-huan,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhou, Jing-Jiang. 2014

[13]A Female-Biased Odorant Receptor from Apolygus lucorum (Meyer-Dur) Tuned to Some Plant Odors. Zhang, Meiping,Zhang, Zhixiang,Yan, Shuwei,Wang, Guirong,Liu, Yang. 2016

[14]Electrophysiological Responses and Reproductive Behavior of Fall Webworm Moths (Hyphantria cunea Drury) are Influenced by Volatile Compounds from Its Mulberry Host (Morus alba L.). Tang, Rui,Zhang, Zhong-Ning,Tang, Rui,Zhang, Feng,Tang, Rui,Zhang, Feng. 2016

[15]Function and Immunocytochemical Localization of Two Novel Odorant-Binding Proteins in Olfactory Sensilla of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae). Deng, Sisi,Yin, Jiao,Zhong, Tao,Cao, Yazhong,Li, Kebin. 2012

[16]Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals. Ren, Li-Yan,Zhang, Yong-Jun,Wu, Kong-Ming,Guo, Yu-Yuan,Li, Ke-Ming. 2012

[17]Electrophysiological and Behavioral Activity of Compounds in Metathoracic Glands of Adults of Tessaratoma Papillosa (Hemiptera: Pentatomidae). Wang, Yujie,Zhao, Dongxiang,Gao, Jinglin,Peng, Zhengqiang,Wang, Yujie,Li, Xiaona,Wang, Yun. 2012

[18]Functional expression analysis of an acyl-ACP thioesterase FatB1 from Arachis hypogaea L. seeds in Escherichia coli. Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,He, Qingfang,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping. 2012

[19]Identification and expression analysis of three c-type lysozymes in Oreochromis aureus. Gao, Feng-ying,Qu, Lan,Ye, Xing,Tian, Yuan-yuan,Zhang, Li-li,Bai, Jun-jie,Lu, Maixin,Qu, Lan,Ye, Xing,Yu, Shao-guo. 2012

[20]The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses. Xiao-Xiao Cheng,Li, Zhi-Fang,Zhu, He-Qin,Li-Hong Zhao,Steven J. Klosterman,Hong-Jie Feng,Zi-Li Feng,Feng Wei,Yong-Qiang Shi,Zhi-Fang Li,He-Qin Zhu.

作者其他论文 更多>>

微信小程序