Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice

文献类型： 外文期刊

第一作者： Zhang, Xiu-Xiang

作者： Zhang, Xiu-Xiang;Zhang, Xiu-Xiang;Yu, Hui;Wang, Xiao-Hu;Luo, Sheng-Jun;Li, Xiu-Zhen;Li, Hao-Xin;Yuan, Zi-Guo;Zhu, Yan-Ping

作者机构：

关键词： MOMP;Transgenic rice;Oral immunization;Edible vaccine

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Avian chlamydiosis is caused by Chlamydophila psittaci (Cp. psittaci) and major outer membrane protein (MOMP) of Cp. psittaci is an excellent vaccine candidate. In this study, the MOMP gene was expressed in rice callus by the Agrobacterium tumefaciensvector. The production of protein in transgenic rice seeds was confirmed and quantified by Western-blot and ELISA, the results demonstrating that the antigen was expressed stably. The transgenic rice seeds expressing the MOMP protein were administered by the oral route to BALB/c mice, which developed MOMP-specific serum IgG and fecal IgA antibodies and a splenocyte MOMP-specific proliferative response and significant levels of IFN-gamma, IL-2, IL-4, IL-5 and TGF-beta production. Immunization with MOMPtransgenic seeds induced partial protection (50%) against a lethal challenge with the highly virulent Cp. psittaci 6BC strain. Lung function after challenge was less affected compared non-MOMP immunized animals. The results demonstrate the feasibility ofusing transgenic rice seeds as an oral vaccine to generate protective immunity and reduce the lung lesions in mice against virulent Cp. psittaci 6BC strain. This finding has implications for further development of an oral vaccine against avian chlamydiosis.

分类号： R392

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