Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl

文献类型: 外文期刊

第一作者: Li, S. M.

作者: Li, S. M.;Ouyang, L. H.;Zhou, D. G.

作者机构:

关键词: vitamin D_3;induction;chicken;avian beta-defensins;real-time PCR

期刊名称:CZECH JOURNAL OF ANIMAL SCIENCE ( 影响因子:1.267; 五年影响因子:1.428 )

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收录情况: SCI

摘要: The expression of avian beta-defensins (AvBDs), Toll-like receptors (TLRs), and vitamin D receptor (VDR) following in vivo vitamin D_3 injection was studied Healthy 90-day Silky Fowls were abdominally injected with vitamin D_3 or untreated. Real-timePCR analyses revealed that injection of vitamin D_3 significantly (P < 0.05) up-regulated the expression of TLRs (TLR2, TLRS), VDR, AvBDs (.AVBD-6, GAL-1), and 24-hydroxylase (CYP24A1) in the tissues (liver, spleen, and kidney) at various times 8-24 h post injection. These results suggest that expression of VDR, AvBDs, and TLRs seems to be induced by vitamin D_3 and it was concluded that the tissues expressing TLRs and VDR respond to vitamin D_3 and in turn upregulate these tissues cellular functions tosynthesize AvBDs. Intraperitoneal injection of vitamin D_3 likely resulted in enhancing the expression of AvBDs, TLRs, and VDR, which provided insight into factors important for the control of the innate immune response in the chickens.

分类号: Q95

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