Molecular cloning and expression of 17 beta-hydroxysteroid dehydrogenase type 2 gene in Hu sheep

文献类型： 外文期刊

第一作者： Wang, Chang Long

作者： Wang, Chang Long;Ying, Shi Jia;Wang, Zi Yu;Xing, Hui Jun;Wang, Li Zhong;He, Dong Yang;Wang, Feng;Wang, Chang Long;Ying, Shi Jia;Wang, Zi Yu;Xing, Hui Jun;Wang, Li Zhong;He, Dong Yang;Wang, Feng;Ying, Shi Jia;Xiao, Shen Hua

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关键词： 17 beta-HSD2;Tissue expression analysis;Hu sheep

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： 17 beta-Hydroxysteroid dehydrogenase type 2 (17 beta-HSD2) catalyzes the NADP+-dependent oxidation of the most potent estrogen 17 beta-estradiol into the weak estrogen estrone, and the conversion of testosterone to androstenedione. It has been reported that 17 beta-HSD2 was expressed in many tissues in human, rats, however, the full-length sequence of 17 beta-HSD2 gene and its expression in ewe were still unknown. In this study, we cloned the full-length cDNA sequence and investigated mRNA differential expression in 28 tissues of 12 adult Hu-Sheep which were fed with high- and low- dietary intake. The 1,317 bp full-length cDNA sequence was first cloned. The coding region was 1,167 bp in length, and the monomer was estimated to contain 389 amino acidresidues. It shares high AA sequence identity with that of bos Taurus (96.13 %), sus scrofa (77.06 %), canis lupus familiaris (70.44 %), Callithrix jacchus (65.72 %), Nomascus leucogenys (65.46 %), pan troglodytes (65.21 %), human (64.69 %), mus musculus (58.35 %), and a comparatively lower identity to danio rerio (37.85 %). 17 beta-HSD2 gene was high expressed in gastrointestinal (GI) tract, liver, but weakly expressed in other tissues. No detected expression was examined in lung. 17 beta-HSD2 gene expression was significantly difference in rumen, omasum, duodenum, cecum, hypophysis after high- and low- dietary intake. Results from the present study suggested that 17 beta-HSD2 plays a crucial role in almost all tissues protecting against excessive levels of active steroid hormone, and GI tract maybe an important steroid hormone metabolizing organ in Hu-Sheep. This present study is the first to provide the primary foundation for further insight into this ovine gene.

分类号： Q7

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