Isolation, characterization, and expression analysis of CmMLO2 in muskmelon

文献类型： 外文期刊

第一作者： Cheng, Hong

作者： Cheng, Hong;Kong, Weiping;Hou, Dong;Tao, Xinglin;Lv, Junfeng

作者机构：

关键词： Cloning;Expression;Melon;MLO

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The full-length cDNA sequence of the MLO gene was cloned via SMART-RACE-PCR from muskmelon (Cucumis melo L.), and was designated as CmMLO2 (GenBank Accession No. FJ713542). The gene is 1,710 bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain topology, and is a typical transmembrane protein. Localization analysis in onion epidermal cells showed that CmMLO2-GFP is localized in the plasma membrane. The expression of CmMLO2 gene was analyzed in melon leaf infected with powderymildew using a quantitative RT-PCR and it was found that CmMLO2 was mainly expressed in melon leaves in a no-tissue-specific pattern. Moreover, CmMLO2 may play a crucial role in the pathogenesis of powdery mildew.

分类号： Q7

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