Electrochemical Behavior of Calcein and the Interaction Between Calcein and DNA

文献类型： 外文期刊

第一作者： Zhang, Xuzhi

作者： Zhang, Xuzhi;Cui, Yi;Zhao, Jun;Cui, Zhengguo;Li, Qiufen;Qu, Keming;Li, Meng

作者机构：

关键词： Calcein;Conductive carbon black paste electrode;DNA interaction;Electrochemical behavior;Voltammetry

期刊名称：ELECTROANALYSIS （ 影响因子：3.223； 五年影响因子：2.947 ）

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收录情况： SCI

摘要： The electrochemical behavior of calcein (CA) has been investigated by using a conductive carbon black paste electrode (CCBPE) as working electrode. It exhibits a single well-defined redox peak in phosphate buffered saline in the range of pH5.5-8.0, which attributes to the irreversible oxidation with 2 electrons and 2 protons participation. Under the optimized analytical conditions, the proposed linear sweep voltammetry (LSV) method allows the determination of CA in a linear concentration range of 0.64-9.60μM, with a limit of detection of 0.32μM. Further, the interaction between CA and DNA were studied by voltammetric and spectrometric methods. Both studies have shown that CA can bind to DNA by the intercalation binding mode. Under the present experimental condition, the binding constant β of CA and dsDNA is 1.10×10 ~7. Meanwhile, in the loop-mediated isothermal amplification (LAMP) reaction mixture there is obvious interaction between CA and dsDNA, resulting in a nonignorable decrease of the indicating sensitivity.

分类号： O646

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