Serological and molecular evidence for natural infection of Bactrian camels with multiple subgenotypes of bovine viral diarrhea virus in Western China

文献类型： 外文期刊

第一作者： Gao, Shandian

作者： Gao, Shandian;Luo, Jihuai;Du, Junzheng;Lang, Yifei;Cong, Guozheng;Shao, Junjun;Lin, Tong;Zhao, Furong;Chang, Huiyun;Yin, Hong;Belak, Sandor;Liu, Lihong

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关键词： Bovine viral diarrhea virus (BVDV);Subgenotype;Bactrian camel (Camelus bactrianus);Western China

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： Bovine viral diarrhea virus (BVDV) infects both domestic and wild animals, causing substantial economic losses. In order to investigate possible infection in Bactrian camels in Western China, a total of 56 blood samples were collected from clinically healthy Bactrian camels and tested for BVDV antigens and antibodies using antigen capture enzyme-linked immunosorbent assay (ELISA) and virus neutralization test. The antigen-positive samples (n = 17) were further tested for viral nucleic acids by species-specific real-time RT-PCR assays, which showed presence of BVDV-1, but not BVDV-2 nor atypical bovine pestivirus, in the camel samples. Twelve non-cytopathogenic viruses were isolated and genetically typed by sequencing of the 5'untranslated region (5'UTR) and N-pro coding sequences. Phylogenetic analysis divided the isolates into six known subgenotypes: BVDV-1a, BVDV-1b, BVDV-1c, BVDV-1m, BVDV-1o, BVDV-1p and a putative subgenotype, BVDV-1q. This study provides, for the first time, serological and molecular evidence for natural infection of Bactrian camels in Western China with highly divergent BVDV-1 strains. Further investigations are needed to elucidate the possible roles of Bactrian camels in the epidemiology of BVD in Western China

分类号： S8

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