An Efficient Strategy Based on Macroporous Resins and Semi-Preparative High Performance Liquid Chromatography for Rapid Separation of Five Flavonoids Components From Flaveria Bidentis(L.) Kuntze
文献类型: 外文期刊
第一作者: Wei, Yun
作者: Wei, Yun;Yin, Li;Xie, Qianqian;Zhang, Guoliang
作者机构:
关键词: flavonoid;macroporous resins;purification;semi-preparative high performance liquid chromatography
期刊名称:SEPARATION SCIENCE AND TECHNOLOGY ( 影响因子:2.475; 五年影响因子:2.077 )
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收录情况: SCI
摘要: In this study, a simple, rapid, and efficient purification method of five major flavonoids from crude Flaveria bidentis extracts was established. Five flavonoids components were initially obtained by ethanol-water extraction of Flaveria bidentis (L.) Kuntze, followed by using D4020 resin-based column chromatography and semi-preparative high performance liquid chromatography. 9.3 mg hyperoside, 6.5 mg patuletin-3-O-glucoside, 1.7 mg isorhamnetin 3-sulphate, 8.4 mg astragalin, and 4.9 mg 6-methoxykaempferol-3-O-galactoside at high purity of over 94% were obtained from 580 mg Flaveria bidentis extracts. This method is more efficient than HSCCC comparing with the results of these two methods regarding analysts' purity, recovery, and running time.
分类号: O65
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