文献类型: 外文期刊
第一作者: Sun, Yingjie
作者: Sun, Yingjie;Ding, Na;Yu, Shengqing;Meng, Chunhun;Chen, Hongjun;Qiu, Xusheng;Zhang, Shilei;Yu, Yang;Zhan, Yuan;Ding, Chan;Ding, Siyu Serena
作者机构:
关键词: Goose RIG-I gene;Innate immunity;Newcastle disease virus (NDV)
期刊名称:MOLECULAR IMMUNOLOGY ( 影响因子:4.407; 五年影响因子:4.227 )
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年卷期:
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收录情况: SCI
摘要: Mammalian retinoic acid-inducible gene I (RIG-I) is a chief antiviral gene sensing viral RNA molecules including Newcastle disease virus (NDV). In this study, goose RIG-I gene (gRIG-I) was identified. The 2805. bp-long gene encodes a gRIG-I protein that exhibits 93.8% amino acid identity to duck RIG-I. DF-1 chicken fibroblast cells transfected with full-length of gRIG-I or CARD domain of gRIG-I plasmids respond significantly to the agonist of 21-mer 5'ppp RNA, evident through enhancement of IFN-?? promoter activity. Goose RIG-I transfected 293T/17 cells were then tested for the response to NDV infection, resulting in up-regulated activity of IFN-?? promoter, and mRNA levels of IRF-3 and IFIT1, but decreased virus titer. Similar results were obtained in transfected DF-1 chicken fibroblast cells and goose embryo fibroblast cells in response to NDV infections Animal experiments further support a role of gRIG-I in goose innate immunity against NDV infections by showing increased gRIG-I mRNA levels and decreased virus titer in geese lung and air sac post-infection. ? 2012 Elsevier Ltd.
分类号: R392
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