Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence

文献类型： 外文期刊

第一作者： Hao, Bin

作者： Hao, Bin;Mo, Zhao-Lan;Xiao, Peng;Pan, Hai-Jian;Lan, Xin;Li, Gui-Yang;Hao, Bin;Pan, Hai-Jian;Lan, Xin;Mo, Zhao-Lan;Li, Gui-Yang

作者机构：

关键词： Vibrio anguillarum;RpoN (σ~(54));Biofilm;Exopolysaccharides;Metalloproteases;Virulence

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： sigma factor σ~(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 ΔrpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3.

分类号： Q939.9

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