Batch and multi-step fed-batch enzymatic saccharification of Formiline-pretreated sugarcane bagasse at high solid loadings for high sugar and ethanol titers

文献类型： 外文期刊

第一作者： Dong, Lei

作者： Dong, Lei;Liu, Dehua;Chen, Liang

作者机构：

关键词： Enzymatic saccharification;Fed-batch process;Formiline pretreatment;High solid loading;Lignocellulose

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Formiline pretreatment pertains to a biomass fractionation process. In the present work, Formiline-pretreated sugarcane bagasse was hydrolyzed with cellulases by batch and multi-step fed-batch processes at 20% solid loading. For wet pulp, after 144. h incubation with cellulase loading of 10. FPU/g dry solid, fed-batch process obtained ~150. g/L glucose and ~80% glucan conversion, while batch process obtained ~130. g/L glucose with corresponding ~70% glucan conversion. Solid loading could be further increased to 30% for the acetone-dried pulp. By fed-batch hydrolysis of the dried pulp in pH. 4.8 buffer solution, glucose concentration could be 247.3. ±. 1.6. g/L with corresponding 86.1. ±. 0.6% glucan conversion. The enzymatic hydrolyzates could be well converted to ethanol by a subsequent fermentation using Saccharomices cerevisiae with ethanol titer of 60-70. g/L. Batch and fed-batch SSF indicated that Formiline-pretreated substrate showed excellent fermentability. The final ethanol concentration was 80. g/L with corresponding 82.7% of theoretical yield.

分类号： Q

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