Production of Tyramine by Enterococcus faecalis Strains in Water-Boiled Salted Duck

文献类型： 外文期刊

第一作者： Liu, Fang

作者： Liu, Fang;Xu, Weiyan;Wang, Daoying;Zhang, Muhan;Zhu, Yongzhi;Xu, Weimin;Du, Lihui

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期刊名称：JOURNAL OF FOOD PROTECTION （ 影响因子：2.077； 五年影响因子：2.224 ）

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收录情况： SCI

摘要： The potential to produce biogenic amines was investigated with 15 Lactococcus lactis and 15 Enterococcus faecalis strains isolated from water-boiled salted duck. The production of biogenic amines from the isolated strains grown in de Man Rogosa Sharpebroth containing precursor amino acids was determined by thin-layer chromatography and high-performance liquid chromatography. None of the L. lactis strains produced any biogenic amines, whereas 12 strains of E. faecalis produced tyramine and P-phenylethylamine. PCR assays were used to detect the presence of tyrosine decarboxylase genes in all of the isolated strains. Only the 12 biogenic amine-producing Enterococcus strains had a 924-bp fragment characteristic for the tyrosine decarboxylase gene. Thecomparison of the amplified partial tyrDC gene sequences of the 12 positive Enterococcus strains revealed 99% similarity within the same species. The tyramine production of the sterilized water-boiled salted duck inoculated with E. faecalis R612Z1 increased significantly during storage. This study reveals that the isolated E. faecalis strains can produce tyramine and P-phenylethylamine in the medium; however, they can only produce tyramine in water-boiled salted duck.

分类号： TS205

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