Purification and Identification of an ACE Inhibitory Peptide from Walnut Protein

文献类型： 外文期刊

第一作者： Liu, Meng

作者： Liu, Meng;Du, Ming;Zhang, Yingchun;Xu, Weili;Zhang, Lanwei;Wang, Cong;Wang, Kejian

作者机构：

关键词： walnut;peptide;ACE-inhibitory activity;purification;characterization

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present study, a novel angiotensin I-converting enzyme (ACE)-inhibitory peptide, P-2a2, was purified to homogeneity from walnut protein hydrolysate by ultrafiltration, consecutive column chromatography, and high-performance liquid chromatography. The purified peptide was characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrophotometry and a liquid-phase peptide sequencer. The molecular mass of P-2a2 was tested to be 1033.42 D. Its amino acid sequence was determined to be Trp-Pro-Glu-Arg-Pro-Pro-Gln-Ile-Pro. The potent ACE-inhibitory peptide is an enneapeptide and shows a high ACE-inhibitory activity, with an IC_(50) value of 25.67 μg/mL.

分类号： R15

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