The methylation of the PcMYB10 promoter is associated with green-skinned sport in max red bartlett pear

文献类型： 外文期刊

第一作者： Wang, Zhigang

作者： Wang, Zhigang;Meng, Dong;Li, Tianzhong;Wang, Zhigang;Jiang, Shuling;Cong, Peihua;Wang, Aide;Li, Tianlai;Wang, Zhigang;Jiang, Shuling;Cong, Peihua

作者机构：

关键词： methylation;associated;bartlett pear

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Varieties of the European pear (Pyrus communis) can produce trees with both red- and green-skinned fruits, such as the Max Red Bartlett (MRB) variety, although little is known about the mechanism behind this differential pigmentation. In this study, we investigated the pigmentation of MRB and its green-skinned sport (MRB-G). The results suggest that a reduction in anthocyanin concentration causes the MRB-G sport. Transcript levels of PcUFGT (for UDP-glucose:flavonoid 3-O-glucosyltransferase), the key structural gene in anthocyanin biosynthesis, paralleled the change of anthocyanin concentration in both MRB and MRB-G fruit. We cloned the PcMYB10 gene, a transcription factor associated with the promoter of PcUFGT. An investigation of the 2-kb region upstream of the ATG translation start site of PcMYB10 showed the regions 2604 to 2911 bp and 21,218 to 21,649 bp to be highly methylated. A comparison of the PcMYB10 promoter methylation level between the MRB and MRB-G forms indicated a correlation between hypermethylation and the green-skin phenotype. An Agrobacterium tumefaciens infiltration assay was conducted on young MRB fruits by using a plasmid constructed to silence endogenous PcMYB10 via DNA methylation. The infiltrated fruits showed blocked anthocyanin biosynthesis, higher methylation of the PcMYB10 promoter, and lower expression of PcMYB10 and PcUFGT. We suggest that the methylation level of PcMYB10 is associated with the formation of the green-skinned sport in the MRB pear. The potential mechanism behind the regulation of anthocyanin biosynthesis is discussed.

分类号： Q945

  • 相关文献

[1]The effects of dietary betaine supplementation on fatty liver performance, serum parameters, histological changes, methylation status and the mRNA expression level of Spot14 alpha in Landes goose fatty liver. Su, S. Y.,Li, Q. F.,Wang, H. W.,Xie, Z.,Su, S. Y.,Wang, H. W.,Li, X. B.,Li, Q. F.,Dodson, M. V.. 2009

[2]Progress in the studies of foreign gene silencing in transgenic plants.. Zhu, L,Zhang, CY,Fan, YL. 1999

[3]Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis). Dong Zhongdian,Qi Qian,Zhang Ning,Wen Haishen,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin. 2016

[4]Comparison of Six Methylation Methods for Fatty Acid Determination in Yak Bone Using Gas Chromatography. Jia, Wei,Wu, Ting,Yu, Qunli,Jia, Wei,Liu, Wenyuan,Mi, Si,Zhang, Chunhui,Li, Xia,Wu, Ting,Yu, Qunli,Liu, Wenyuan,Yu, Qunli.

[5]Characterization and expression of cyp19a gene in the Chinese giant salamander Andrias davidianus. Hu, Qiaomu,Xiao, Hanbing,Tian, HaiFeng,Meng, Yan,Hu, Qiaomu.

[6]An Integrated Analysis of miRNAs and Methylated Genes Encoding mRNAs and lncRNAs in Sheep Breeds with Different Fecundity. Miao, Xiangyang,Luo, Qingmiao,Zhao, Huijing,Qin, Xiaoyu. 2017

[7]Paternal Fenvalerate Exposure Influences Reproductive Functions in the Offspring. Xia, Dong,Xu, Kesi,Jiang, Hui,Li, Rongjie,Hang, Yiqiong,Lu, Yang,Parvizi, Nahid,Zhou, Yuchuan. 2013

[8]Argininosuccinate synthetase gene is silenced by CpG methylation in children with phenylketonuria. Li, Li,Shao, GenZe,Lin, Ming,Jin, ChunMei,Ye, LiTao,Wang, LiDe. 2013

[9]Recombination in diverse maize is stable, predictable, and associated with genetic load. Rodgers-Melnick, Eli,Bradbury, Peter J.,Elshire, Robert J.,Glaubitz, Jeffrey C.,Acharya, Charlotte B.,Mitchell, Sharon E.,Buckler, Edward S.,Bradbury, Peter J.,Buckler, Edward S.,Li, Chunhui,Li, Yongxiang.

[10]Characterization of the NLRC5 promoter in chicken: SNPs, regulatory elements and CpG islands. Guo, X. M.,Chang, G. B.,Xu, L.,Bi, Y. L.,Zhang, Y.,Zhu, P. F.,Wu, Y.,Chen, G. H.,Liu, X. P.,Wang, H. Z..

[11]Comparison of the heat stress induced variations in DNA methylation between heat-tolerant and heat-sensitive rapeseed seedlings. Gao, Guizhen,Li, Jun,Li, Hao,Li, Feng,Xu, Kun,Yan, Guixin,Chen, Biyun,Qiao, Jiangwei,Wu, Xiaoming.

[12]Arsenate reduction and methylation in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 investigated with X-ray absorption near edge structure. Su, S. M.,Zeng, X. B.,Li, L. F.,Duan, R.,Bai, L. Y.,Li, A. G.,Wang, J.,Jiang, S.. 2012

[13]Simplified Method for Determination of Mancozeb Residues in Apple Using Ultra Performance Liquid Chromatography-Mass Spectrometry. Dong Feng-Shou,Liu Xin-Gang,Cheng Li,Wang Chen-Rui,Zheng Yong-Quan,Chen Wu-Ying,Liao Xiao-Lan,Qin Dong-Mei. 2010

[14]Targeted BSA mapping of Scmv1 and Scmv2 conferring resistance to SCMV using PstI/MseI compared with EcoRI/MseI AFLP markers. Yuan, L,Dussle, CM,Muminovic, J,Melchinger, AE,Lubberstedt, T. 2004

[15]Effect of 5-aza-2 '-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Wang, Dong,Liu, Yan,Qin, Tong,Zhu, Hua-Bin. 2012

[16]The Effect of the Cyp19a1 Gene Methylation Modification on Temperature-dependent Sex Determination of Reeves' Turtle (Mauremys reevesii). Ru, Wenxiu,Chen, Hong,Lan, Xianyong,Ru, Wenxiu,Hua, Liushuai,Wei, Yufeng,Li, Weiye,Cao, Dainan,Ge, Yan,Gong, Shiping,Hua, Liushuai. 2017

[17]Structural elucidation and antioxidant activity of a novel polysaccharide (TAPB1) from Tremella aurantialba. Du, Xiuju,Zhang, Yang,Lv, Zhiwei,Yang, Yan,Zhang, Jingsong,Mu, Hongmei.

[18]Duplicated CCAAT/enhancer-binding protein beta (C/EBP beta) gene: Transcription and methylation changes in response to dietary betaine in Landes goose liver. Yu, S. L.,Li, Q. F.,Xie, Z.,Su, S. Y.,Zhang, X.. 2013

[19]Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress. Fang, Yujie,Li, Jian,Jiang, Jinjin,Geng, Yulu,Wang, Jinglei,Wang, Youping,Fang, Yujie.

[20]Characterization of Growth and Reproduction Performance, Transgene Integration, Expression, and Transmission Patterns in Transgenic Pigs Produced by piggyBac Transposition-Mediated Gene Transfer. Zeng, Fang,Li, Zicong,Gao, Wenchao,Jiang, Gelong,Liu, Dewu,Wu, Zhenfang,Zeng, Fang,Li, Zicong,Gao, Wenchao,Jiang, Gelong,Liu, Dewu,Wu, Zhenfang,Cai, Gengyuan,Urschitz, Johann,Moisyadi, Stefan,Moisyadi, Stefan.

作者其他论文 更多>>

微信小程序