Sex determination of bovine preimplantation embryos by oligonucleotide microarray

文献类型: 外文期刊

第一作者: Yang, Hua

作者: Yang, Hua;Zhong, Fagang;Yang, Yonglin;Wang, Xinhua;Liu, Shouren;Zhu, Bin

作者机构:

关键词: Oligonucleotide microarray;Sex determination;SRY gene;Bovine embryos

期刊名称:ANIMAL REPRODUCTION SCIENCE ( 影响因子:2.145; 五年影响因子:2.281 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The aim has been to set up a rapid and accurate microarray assay using sandwich mode for sex determination of bovine preimplantation embryos. Twelve sequence-specific oligonucleotide capture probes used to discriminate 12 samples were spotted onto thealdehyde-modified glass slides by Arrayer. The 2 recognition probes used to identify coding regions of the sex-determining region of the Y chromosome gene (SRY) and (3-casein (CSN2) reference gene were coupled with biotin. The assay was optimized by using genomic DNA extracted from blood samples of known sex individuals. Polymerase chain reaction (PCR) was used to amplify the fragments in the HMG box region of SRY gene and CSN2 gene with sequence-specific primers. The sex of samples was identified by detecting both the SRY and CSN2 genes simultaneously in 2 reaction cells of microarrays, with the male having SRY and CSN2 signals and the female only CSN2. The sex of 20 bovine preimplantation embryos was determined by oligonucleotide microarray. The protocol was run with a blind test that showed a 100% (82/82) specificity and accuracy in sexing of leukocytes. The bovine embryos were transferred into 20 bovine recipients, with a pregnant rate of 40% (8/20). Three calves were born at term, and 5 fetuseswere miscarried. Their sexes were fully in accordance with the embryonic sex predetermination predicted by oligonucleotide microarray. This suggests that the oligonucleotide microarray method of SRY gene analysis can be used in early sex prediction of bovine embryos in breeding programs.

分类号: Q95

  • 相关文献

[1]Sex determination by SRY PCR and sequencing of Tasmanian devil facial tumour cell lines reveals non-allograft transmission. Cui, Xianlan,Hua, Bobby,Wang, Yunfeng,Zhao, Yan,Cui, Hongyu,Kong, Xiangang,Miller, Webb.

[2]Differential Gene Expression Profiles in Human THP-1 Monocytes Treated with Lactobacillus plantarum or Staphylococcus aureus Lipoteichoic Acid. Zeng, Ri-Zhong,Kim, Han Geun,Kim, Na Ra,Geun, Min,Chung, Dae Kyun,Zeng, Ri-Zhong,Kim, Han Geun,Kim, Na Ra,Geun, Min,Chung, Dae Kyun,Ko, Mi Yeon,Lee, Seung Yeon,Chung, Dae Kyun,Kim, Chul Min,Chung, Dae Kyun,Zeng, Ri-Zhong,Chung, Dae Kyun.

[3]APPLICATION OF OLIGONUCLEOTIDE MICROARRAY FOR THE DETECTION AND GENOTYPING OF CRY GENES IN Bacillus thuringiensis. Liu Xu-Guang,Song Fu-Ping,Zhang Jie,Liu Xu-Guang,Wen Si-Yuan,Wang Sheng-Qi,Huang Da-Fang. 2010

[4]Sex-biased miRNAs of yellow catfish (Pelteobagrus fulvidraco) and their potential role in reproductive development. Wang, Pingping,Wang, Le,Yang, Jian,Luan, Peixian,Zhang, Xiaofeng,Kuang, Youyi,Sun, Xiaowen. 2018

[5]Mutation in the gene encoding 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4) led to andromonoecy in watermelon. Ji, Gaojie,Zhang, Jie,Zhang, Haiying,Sun, Honghe,Gong, Guoyi,Shi, Jianting,Tian, Shouwei,Guo, Shaogui,Ren, Yi,Xu, Yong,Ji, Gaojie,Shen, Huolin,Gao, Junping. 2016

[6]Construction of a high-density linkage map and mapping of sex determination and growth-related loci in the mandarin fish (Siniperca chuatsi). Sun, Chengfei,Ye, Xing,Dong, Junjian,Zeng, Qingkai,Chen, Zhihang,Tian, Yuanyuan,Lu, Maixin,Niu, Yongchaox,Hu, Wushu,Zeng, Qingkai,Chen, Zhihang,Tian, Yuanyuan,Zhang, Jin. 2017

[7]Key Signaling Events for Committing Mouse Pluripotent Stem Cells to the Germline Fate. Wang, Jian-Qi,Cao, Wen-Guang.

[8]Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties. Liu, Yating,Liu, Yating,Xie, Wen,Yang, Xin,Guo, Litao,Wang, Shaoli,Wu, Qingjun,Yang, Zezhong,Zhang, Youjun,Zhou, Xuguo.

[9]Sex-lethal gene of the Chinese mitten crab Eriocheir sinensis: cDNA cloning, induction by eyestalk ablation, and expression of two splice variants in males and females. Shen, Huaishun,Hu, Yacheng,Zhou, Xin,Shen, Huaishun,Hu, Yacheng.

[10]Establishment and characterization of fibroblast cell line of the Songliao Black pig. Liu, Xuedong,Li, Lin-Feng,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Xuedong,Li, Lin-Feng.

[11]Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense. Zhang, Y. P.,Zhang, W. Y.,Fu, H. T.,Zhang, Y. P.,Qiao, H.,Sun, S. M.,Jiang, S. F.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.. 2013

[12]Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro. Salabi, Fatemeh,Cao, Wen G.,Nazari, Mahmood. 2014

[13]Transcriptomic Analysis for Different Sex Types of Ricinus communis L. during Development from Apical Buds to Inflorescences by Digital Gene Expression Profiling. Tan, Meilian,Xue, Jianfeng,Wang, Lei,Fu, Chunling,Yan, Xingchu,Huang, Jiaxiang. 2016

[14]QTL mapping of subgynoecy in cucumber (Cucumis sativus L.). Chen, H.,Shi, Q.,Liu, S.,Wu, J.,Huang, S.,Liu, S.. 2012

[15]Flower Development and Sex Determination between Male and Female Flowers in Vernicia fordii. Mao, Yingji,Liu, Wenbo,Chen, Xue,Lu, Weili,Hou, Jinyan,Ni, Jun,Wang, Yuting,Wu, Lifang,Mao, Yingji,Liu, Wenbo,Chen, Xue,Lu, Weili,Wang, Yuting,Wu, Lifang,Xu, Yang,Lu, Weili,Wang, Yuting. 2017

[16]Use of the non-electrophoretic method to detect testis specific protein gene for sexing in preimplantation bovine embryos. Huang Jinming,You Wei,Wu Naike,Tan Xiuwen. 2007

[17]An ACC Oxidase Gene Essential for Cucumber Carpel Development. Cui, Qingzhi,Sun, Jinjing,Li, Shuai,Zhang, Huimin,Wang, Huaisong,Lin, Tao,Gao, Dongli,Wang, Shenhao,Zhang, Zhonghua,Huang, Sanwen,Zhang, Huimin,Lin, Tao,Gao, Dongli,Huang, Sanwen,Xin, Fengjiao,Li, Xia,Wang, Donghui,Xu, Zhihong. 2016

[18]Construction of Two BAC Libraries from Half-Smooth Tongue Sole Cynoglossus semilaevis and Identification of Clones Containing Candidate Sex-Determination Genes. Shao, Chang-Wei,Chen, Song-Lin,Xu, Jian-Yong,Sha, Zhen-Xia,Dong, Xiao-Li,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin.

[19]Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Xu, Jian-Yong,Liu, Ben-Wei,Deng, Si-Ping.

[20]Molecular analysis shows differential expression of R-spondin1 in zebrafish (Danio rerio) gonads. Zhang, Yanmei,Li, Fei,Liu, Jiangdong,Liu, Na,Yu, Qixing,Sun, Dongchang.

作者其他论文 更多>>