Rapid diagnosis of goose viral infections by multiplex PCR

文献类型: 外文期刊

第一作者: Chen, Zongyan

作者: Chen, Zongyan;Li, Chuanfeng;Li, Guoxin;Yu, Hai;Jiang, Yifeng;Yan, Liping;Meng, Chunchun;Zhou, Yanjun;Tong, Guangzhi;Liu, Guangqing

作者机构:

关键词: Goose parvovirus;Newcastle disease virus;Goose herpesvirus;Goose adenovirus;Multiplex PCR

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

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收录情况: SCI

摘要: Goose parvovirus (GPV), newcastle disease virus (NDV), goose herpesvirus (GHV) and goose adenovirus (GAV) are considered collectively to be four of the most important and widespread viruses of geese. Because all of these viruses cause similar pathological changes, histological differentiation among these viruses is difficult. A reliable, specific and sensitive multiplex PCR (mPCR) assay was developed for the combined detection of GPV, NDV, GHV and GAV in clinical samples of geese. Using the mPCR technique, single infections with GPV (28/76; 36.8%), NDV (9/76; 11.8%), GHV (3/76; 3.9%) and GAV (12/76; 15.8%) were identified in the samples; co-infections with GAV and either GPV or NDV (31.6%; 24/76) were also identified with this approach. The resultsfor all of the samples tested were the same in both the uPCR and mPCR systems. The mPCR approach is considered to be useful for routine molecular diagnosis and epidemiological applications in geese.

分类号: R37

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