Complete genome sequence and molecular characterization of thermostable newcastle disease virus strain Ts09-C
文献类型: 外文期刊
第一作者: Wen, Guoyuan
作者: Wen, Guoyuan;Shao, Huabin;Luo, Qingping;Yang, Jun;Wang, Hongling;Wen, Guoyuan;Shang, Yu;Guo, Jing;Chen, Chen;Cheng, Guofu
作者机构:
关键词: Complete genome sequence;Newcastle disease virus;Strain TS09-C;Thermostable
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
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收录情况: SCI
摘要: A thermostable avirulent Newcastle disease virus (NDV) strain TS09-C was developed by serial passage of V4 strain in BHK-21 cells. The complete genome sequence of strain TS09-C was determined and compared with the sequences of other NDV isolates representing different thermostable phenotypes. The TS09-C genome was 15,186 nucleotides long and consisted of eight genes in the order of 3′-NP-P/V/W-M-F-HN-L-5′. High levels of nucleotide and amino acid sequence identity existed among the thermostable NDV isolates. Thermostable strains TS09-C, V4, I-2, and Ulster all belonged to genotype I. The F protein cleavage site of strain TS09-C was 112G-K-Q-R-R-L117, with an isolated basic amino acid and a pair of contiguous basic amino acids, differing from that of its parental V4 strain (112G-K-Q-G-R-L 117). Our characterization of the complete genome of thermostable avirulent NDV strain TS09-C may facilitate the development of a thermostable NDV reverse genetic system and further study of the mechanism of thermostability of NDV.
分类号: R37
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