Codon optimization and woodchuck hepatitis virus posttranscriptional regulatory element enhance the immune responses of DNA vaccines against infectious bursal disease virus in chickens

文献类型： 外文期刊

第一作者： Li, Kai

作者： Li, Kai;Gao, Li;Gao, Honglei;Qi, Xiaole;Gao, Yulong;Qin, Liting;Wang, Yongqiang;Wang, Xiaomei

作者机构：

关键词： Codon optimization;DNA vaccine;Infectious bursal disease virus;WPRE

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The present study was undertaken to evaluate the protective efficacy of DNA vaccines against infectious bursal disease virus (IBDV) in chickens and to determine whether codon optimization and the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) could improve the immunogenicity of the DNA vaccines. The VP2, VP243 and codon-optimized VP243 genes of IBDV were cloned into pCAGGS vector, and designated as pCAGVP2, pCAGVP243 and pCAGoptiVP243, respectively. Plasmids pCAGWVP243 and pCAGWoptiVP243 carrying the WPRE elements were also constructed as DNA vaccines. To evaluate vaccine efficacy, 2-week-old chickens were injected intramuscularly with the constructed plasmids twice at 2-week intervals and challenged with very virulent IBDV 2 weeks post-boost. Plasmid pCAGVP243 induced better immune responses than pCAGVP2. Chickens immunized with pCAGoptiVP243 and pCAGWVP243 had higher levels of antibody titers, lymphoproliferation responses and cytokine production compared with pCAGVP243. Furthermore, plasmid pCAGWoptiVP243 induced the highest levels of immune responses among the groups. After challenged, DNA vaccines pCAGVP2, pCAGVP243, pCAGoptiVP243, pCAGWVP243 and pCAGWoptiVP243 conferred protection for 33%, 60%, 80%, 87% and 100% of chickens, respectively, as evidenced by the absence of clinical signs, mortality, and bursal atrophy. These results indicate that codon optimization and WPRE could enhance the protective efficacy of DNA vaccines against IBDV and these two approaches could work together synergistically in a single DNA vaccine.

分类号： R37

  • 相关文献

[1]Protection of chickens against reticuloendotheliosis virus infection by DNA vaccination. Li, Kai,Gao, Li,Gao, Honglei,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei. 2013

[2]Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector. Jiang, Yongping,Yu, Kangzhen,Zhang, Hongbo,Zhung, Pingjing,Li, Chenjun,Tian, Guobin,Li, Yanbing,Wang, Xijun,Ge, Jinying,Bu, Zhigao,Chen, Hualan. 2007

[3]DNA prime-protein boost vaccination enhances protective immunity against infectious bursal disease virus in chickens. Gao, Honglei,Li, Kai,Gao, Li,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei.

[4]Adjuvant effects of interleukin-18 in DNA vaccination against infectious bursal disease virus in chickens. Li, Kai,Gao, Honglei,Gao, Li,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei. 2013

[5]Non-uniqueness of factors constraint on the codon usage in Bombyx mori. Jia, Xian,Zheng, Hao,Qi, Qi,Wei, Lei,Sun, Jingchen,Jia, Xian,Zheng, Hao,Qi, Qi,Wei, Lei,Sun, Jingchen,Liu, Shuyu,He, Jian,Li, Bo,Zhao, Taiyi. 2015

[6]High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization. Gao, Zhaowei,Zhang, Yuhong,Li, Mu,Zhou, Liwei,Zhang, Wei,Gao, Zhaowei,Tang, Yunming,Li, Zhuofu,Huang, Huoqing,Yao, Bin. 2012

[7]Improved secretory production of calf prochymosin by codon optimization and disruption of PMR1 in Kluyveromyces lactis GG799. Feng, Zhen,Ren, Jing,Zhang, Yanhe,Hu, Sen. 2011

[8]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[9]Formation of virus-like particles from O-type foot-and-mouth disease virus in insect cells using codon-optimized synthetic genes. Cao, Yimei,Sun, Pu,Fu, Yuanfang,Bai, Xingwen,Tian, Feipen,Liu, Xiangtao,Lu, Zengjun,Liu, Zaixin.

[10]High level expression of an acid-stable phytase from citrobacter freundii in pichia pastoris. Zhao, Wei,Xiong, Aisheng,Fu, Xiaoyan,Gao, Feng,Tian, Yongsheng,Peng, Rihe.

[11]High yield expression of duck hepatitis A virus VP1 protein in Escherichia coli, and production and characterization of polyclonal antibody. Li, Chuanfeng,Chen, Zongyan,Meng, Chunchun,Li, Lu,Liu, Guangqing. 2013

[12]Chloroplast-targeted expression of the codon-optimized truncated cry1Ah gene in transgenic tobacco confers a high level of protection against insects. Huang, Dafang,Li, Xiuying,Li, Shengyan,Lang, Zhihong,Zhu, Li,Huang, Dafang,Li, Shengyan,Zhang, Jie. 2013

[13]Immunogenicity of virus-like particles containing modified goose parvovirus VP2 protein. Chen, Zongyan,Li, Chuanfeng,Zhu, Yingqi,Wang, Binbin,Meng, Chunchun,Liu, Guangqing.

[14]Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells. Gao, Jingpeng,Meng, Chunchun,Chen, Zongyan,Li, Chuanfeng,Liu, Guangqing.

[15]Overexpression of D-amino acid oxidase from Bradyrhizobium japonicum, enhances resistance to glyphosate in Arabidopsis thaliana. Han, Hongjuan,Zhu, Bo,Fu, Xiaoyan,You, Shuanghong,Wang, Bo,Li, Zhenjun,Zhao, Wei,Peng, Rihe,Yao, Quanhong,You, Shuanghong,Yao, Quanhong.

[16]Cell-Free Expression, Purification, and Characterization of the Functional beta(2)-Adrenergic Receptor for Multianalyte Detection of beta-Agonists. Wang, Jian,Liu, Yuan,Han, Zhengzheng,Wang, Wei,Liu, Yang,Wei, Dong,Wang, Jian,Liu, Yuan,Zhang, Junhua,Huang, Wei. 2017

[17]Cloning, codon-optimized expression and homology modeling of structural protein VP1 from foot and mouth disease virus. Liu, Xin-sheng,Wang, Yong-lu,Zhang, Yong-guang,Fang, Yu-zhen,Pan, Li,Lue, Jian-liang,Zhou, Peng,Zhang, Zhong-wang,Cheng Qi-wei,Wang, Gang,Wang, Ji-wei,Lou, Hui,Jiang, Shou-tian. 2011

[18]Porcine reproductive and respiratory syndrome virus ORF5a protein is essential for virus viability. Lin, Tao,Huang, Ting,Yao, Huochun,Fan, Hongjie,Sun, Lichang,Li, Yanhua,Liu, Runxia,Gao, Fei,Tong, Guangzhi,Wei, Zuzhang,Yuan, Shishan,Li, Yanhua,Wang, Xiaomin.

[19]Extracellular expression and antiviral activity of a bovine interferon-alpha through codon optimization in Pichia pastoris. Tu, Yabin,Wang, Gang,Wang, Yanqun,Chen, Weiye,Zhang, Lu,Liu, Yonggang,Jiang, Chenggang,Wang, Shujie,Bu, Zhigao,Cai, Xuehui.

[20]Characterization and high expression of recombinant Ustilago maydis xylanase in Pichia pastoris. Han, Hongjuan,You, Shuang,Zhu, Bo,Fu, Xiaoyan,Sun, Baihui,Qiu, Jin,Yu, Chengye,Chen, Lei,Peng, Rihe,Yao, Quanhong.

作者其他论文 更多>>

微信小程序