Detection of specific antibodies against tembusu virus in ducks by use of an E protein-based enzyme-linked immunosorbent assay
文献类型： 外文期刊
第一作者： Yin, Xiuchen
作者： Yin, Xiuchen;Lv, Rang;Chen, Xiaodan;Liu, Ming;Hua, Ronghong;Zhang, Yun
作者机构：
期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 影响因子：5.948； 五年影响因子：5.345 ）
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年卷期：
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收录情况： SCI
摘要： We developed an enzyme-linked immunosorbent assay (ELISA) using eukaryotically expressed E protein as the antigen (termed E-ELISA) to detect antibodies to tembusu virus (TMUV) in ducks. The E-ELISA did not react with antisera to other known pathogens, indicating the E protein is specific for recognizing anti-TMUV antibodies. Compared to the serum neutralization test, the specificity and sensitivity of the E-ELISA was 93.2 and 97.8%, respectively. Therefore, this E-ELISA is a sensitive and rapid method for detecting antibodies against TMUV in ducks.
分类号： R37
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