Comparison of genomes of brucella melitensis m28 and the b. melitensis m5-90 derivative vaccine strain highlights the translation elongation factor tu gene tuf2 as an attenuation-related gene

文献类型： 外文期刊

第一作者： Wang, Fangkun

作者： Wang, Fangkun;Qiao, Zujian;Hu, Sen;Liu, Wenxing;Bu, Zhigao;Wang, Fangkun;Liu, Sidang;Zhao, Xiaomin;Zheng, Huajun

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期刊名称：INFECTION AND IMMUNITY （ 影响因子：3.441； 五年影响因子：3.702 ）

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收录情况： SCI

摘要： Brucella melitensis causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. Attenuated B. melitensis strain M5-90, derived from virulent strain M28, is widely used as a live vaccine in ruminants in China. Genetic differences between the strains may cast light on the mechanism of attenuation. We recently reported the complete genomic sequences of M28 and M5-90. Genome organization is highly conserved between these isolates, and also with virulent strains 16 M and ATCC 23457. Analysis revealed 23 open reading frames (ORFs) with consistent differences between M5-90 and the virulent strains. Notably, the tuf2 gene encoding translation elongation factor EF-Tu from M5-90 contained 50 single nucleotide polymorphisms (SNPs) and 9 gaps (indels) compared to tuf2 of M28 or of the other virulent strains. There were no changes in tuf1. To evaluate the potential role of EF-Tu in pathogenesis, tuf1 and tuf2 mutants of M28 and an M5-90 strain harboring wild-type tuf2 were constructed, and their virulence/attenuation was evaluated in vivo. We report that the tuf2 gene plays an important role in the attenuation of M5-90 virulence.

分类号： R51

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