Novel aspects of understanding molecular working mechanisms of salivary glands of worker honeybees (Apis mellifera) investigated by proteomics and phosphoproteomics

文献类型： 外文期刊

第一作者： Feng, Mao

作者： Feng, Mao;Fang, Yu;Han, Bin;Zhang, Lan;Lu, Xiaoshan;Li, Jianke

作者机构：

关键词： 2-DE;Honeybee;Phosphoproteome;Proteome;Salivary gland;Single-cohort colony

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Honeybee salivary glands (SGs) are important exocrine glands. However, the molecular basis of how SGs fulfill their biological duty is still elusive. Proteomics and phosphoproteomics of cephalic SG (HSG) and thoracic SG (TSG) were compared between normal and single-cohort honeybee colonies. Of 113 and 64 differentially regulated proteins and phosphoproteins, 86 and 33 were identified, respectively. The SGs require a wide spectrum of proteins to support their multifaceted functions and ensure normal social management of the colony. Changes of protein expression and phosphoproteins are key role players. The HSG triggers labor transition from in-hive work to foraging activities via the regulation of juvenile hormone and ethyl oleate levels. The stronger expression of proteins involved in carbohydrate and energy metabolism, protein folding, protein metabolism, cellular homeostasis and cytoskeleton in TSG, supports the gland to efficiently enhance honey processing by synthesis and secretion of saliva into nectar. The age structure of the colony is vital for increased production efficiency. This data reveals the molecular underpinning of SGs to accomplish their biological missions and provides new knowledge for the beekeeping industry for enhancing the management and production efficiency of the colony and honey quality through manipulation of potential target proteins. Biological significance: This study comprehensively analyses the characteristic of the proteome and phosphoproteome of honeybee salivary glands (SGs) between normal and single-cohort honeybee colonies. The SGs need a wide spectrum of proteins to support their multifaceted functions and ensure normal social management of the colony. The cephalic SG triggers labor transition from in-hive work to foraging behavior via the regulation of juvenile hormone and ethyl oleate titer. The thoracic SG stronger expressed of proteins related to carbohydrate and energy metabolism, protein folding, protein metabolism, cellular homeostasis and cytoskeleton to support the gland to efficiently enhance honey processing by synthesis and secretion of saliva into nectar. This data reveals the molecular underpinning of SGs to accomplish their biological missions and provides new knowledge for the beekeeping industry for enhancing the maintenance and production efficiency of the colony and honey quality through manipulation of potential target proteins.

分类号： Q51

  • 相关文献

[1]Proteome and phosphoproteome analysis of honeybee (Apis mellifera) venom collected from electrical stimulation and manual extraction of the venom gland. Li, Rongli,Zhang, Lan,Fang, Yu,Han, Bin,Lu, Xiaoshan,Zhou, Tiane,Feng, Mao,Li, Jianke,Zhang, Lan. 2013

[2]Changes of proteome and phosphoproteome trigger embryo-larva transition of honeybee worker (Apis mellifera ligustica). Gala, Alemayehu,Fang, Yu,Woltedji, Dereje,Zhang, Lan,Han, Bin,Feng, Mao,Li, Jianke.

[3]An integrated proteomics reveals pathological mechanism of honeybee (Apis cerena) sacbrood disease. Han, Bin,Zhang, Lan,Feng, Mao,Fang, Yu,Li, Jianke.

[4]Phosphoproteomic Analysis of Protein Phosphorylation Networks in the Hypopharyngeal Gland of Honeybee Workers (Apis mellifera ligustica). Qi, Yuping,Fan, Pei,Hao, Yue,Han, Bin,Fang, Yu,Feng, Mao,Cui, Ziyou,Li, Jianke,Fan, Pei,Cui, Ziyou,Cui, Ziyou.

[5]Brain Membrane Proteome and Phosphoproteome Reveal Molecular Basis Associating with Nursing and Foraging Behaviors of Honeybee Workers. Han, Bin,Fang, Yu,Feng, Mao,Hu, Han,Hao, Yue,Ma, Chuan,Huo, Xinmei,Meng, Lifeng,Zhang, Xufeng,Wu, Fan,Li, Jianke.

[6]The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins. Chao, Qing,Gao, Zhi-fang,Wang, Yue-feng,Mei, Ying-chang,Zhao, Biligen-gaowa,Wang, Bai-chen,Li, Zhe,Huang, Xia-he,Wang, Ying-chun,Li, Liang,Jiang, Yu-bo.

[7]Differential expressions of nuclear proteomes between honeybee (Apis mellifera L.) queen and worker larvae: A deep insight into caste pathway decisions. Begna, Desalegn,Han, Bin,Feng, Mao,Fang, Yu,Li, Jianke.

[8]Proteome Analysis Unravels Mechanism Underling the Embryogenesis of the Honeybee Drone and Its Divergence with the Worker (Apis mellifera lingustica). Fang, Yu,Feng, Mao,Han, Bin,Qi, Yuping,Hu, Han,Fan, Pei,Huo, Xinmei,Meng, Lifeng,Li, Jianke.

[9]Western honeybee drones and workers (Apis mellifera ligustica) have different olfactory mechanisms than eastern honeybees (Apis cerana cerana). Woltedji, Dereje,Song, Feifei,Zhang, Lan,Gala, Alemayehu,Han, Bin,Feng, Mao,Fang, Yu,Li, Jianke.

[10]Mitochondrial proteins differential expression during honeybee (Apis mellifera L.) queen and worker larvae caste determination. Begna, Desalegn,Fang, Yu,Feng, Mao,Li, Jianke.

[11]Differential antennal proteome comparison of adult honeybee drone, worker and queen (Apis mellifera L.). Fang, Yu,Song, Feifei,Zhang, Lan,Aleku, Dereje Woltedji,Han, Bin,Feng, Mao,Li, Jianke.

[12]Proteome Analysis of Hemolymph Changes during the Larval to Pupal Development Stages of Honeybee Workers (Apis mellifera ligustica). Woltedji, Dereje,Fang, Yu,Han, Bin,Feng, Mao,Li, Rongli,Lu, Xiaoshan,Li, Jianke. 2013

[13]Proteomic analysis of responsive root proteins of Fusarium oxysporum-infected watermelon seedlings. Zhang, Man,Xu, Jinhua,Liu, Guang,Yao, Xiefeng,Ren, Runsheng,Yang, Xingping. 2018

[14]Modulation of protein expression in alfalfa (&ITMedicago sativa&IT L.) root and leaf tissues by &ITFusarium proliferatum&IT. Cong Li-li,Long Rui-cai,Kang Jun-mei,Zhang Tie-jun,Wang Zhen,Yang Qing-chuan,Sun Yan,Li Ming-na,Cong Li-li. 2017

[15]Unraveling molecular mechanistic differences in liver metabolism between lean and fat lines of Pekin duck (Anas platyrhynchos domestica): A proteomic study. Zheng, Aijuan,Chang, Wenhuan,Zhang, Shu,Cai, Huiyi,Chen, Guilan,Lou, Ruiying,Liu, Guohua,Hou, Shuisheng.

[16]Drought stress impact on leaf proteome variations of faba bean (Vicia faba L.) in the Qinghai-Tibet Plateau of China. Li, Ping,Wu, Xuexia,Liu, Yujiao,Li, Ping,Liu, Yujiao,Zhang, Yanxia,Li, Ping,Liu, Yujiao. 2018

[17]Proteomic analysis of the phenotype of the scaleless wings mutant in the silkworm, Bombyx mori. Shi, Xiao-Feng,Han, Bin,Li, Yi-Nu,Zhang, Zhi-Fang,Han, Bin,Yi, Yong-Zhu,Shen, Xing-Jia,Li, Xiao-Ming.

[18]Identification of a cysteine-rich antimicrobial peptide from salivary glands of the tick Rhipicephalus haemaphysaloides. Zhang, Houshuang,Zhang, Wenjie,Wang, Xinzhi,Zhou, Yongzhi,Wang, Na,Zhou, Jinlin. 2011

[19]Proteomic Analysis of Proteins in the Salivary Glands of the Fed and Unfed Female Tick Rhipicephalus haemaphysaloides. Xiang Fei-yu,Zhang Jian-wu,Zhou Yong-zhi,Li Zhuang,Gong Hai-yan,Zhou Jin-lin,Xiang Fei-yu. 2009

[20]Identification and anticoagulant activity of a novel Kunitz-type protein HA11 from the salivary gland of the tick Hyalomma asiaticum. Zhang, Houshuang,Qiao, Ruiqiong,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin.

作者其他论文 更多>>

微信小程序