Simultaneous enantioselective determination of triazole fungicide difenoconazole and its main chiral metabolite in vegetables and soil by normal-phase high-performance liquid chromatography

文献类型： 外文期刊

第一作者： Dong, Fengshou

作者： Dong, Fengshou;Cheng, Youpu;Liu, Xingang;Xu, Jun;Li, Yuanbo;Chen, Xiu;Kong, Zhiqiang;Zheng, Yongquan;Cheng, Youpu

作者机构：

关键词： Chiral high-performance liquid chromatography;Chiral metabolite;Difenoconazole;Enantioselective separation;Stereoisomer

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Herein is reported, for the first time, a simple and highly sensitive chiral high-performance liquid chromatography (HPLC) method for the simultaneous quantitative determination of difenoconazole stereoisomers and their hydroxylated metabolite difenoconazole alcohol (CGA- 205375) enantiomers in vegetables and soil matrix. The separation of difenoconazole and CGA-205375 including their simultaneous enantioseparation was studied using four different polysaccharide-type chiral stationary phases (CSPs) in combination with n-hexane-polar organic alcohols mobile phase. Chiralcel OJ consisting of 25 % of cellulose tris(4- methylbenzoate) coated on wide-pore polysaccharide silica gel exhibited higher resolving ability compared to cellulose tris(3,5- dimethylphenylcarbamate) (Chiralcel OD) as well as to its similar amylose derivative (Chiralpak AD) CSPs for this particular set of chiral analytes. Baseline separation and simultaneous enantioseparation of difenoconazole and its metabolite CGA-205375 could be achieved under optimized separation conditions. Based on the established HPLC method, enantioselective analysis method for this fungicide and its main chiral metabolite in vegetables and soil matrix were developed and validated. Parameters including the matrix effect, linearity, precision, accuracy, and stability were evaluated. Under the optimal conditions, the mean recoveries from cucumber, tomato, and soil matrix ranged from 81.65 to 94.52 %, with relative standard deviations in the range of 1.05-8.32 % for all stereoisomers. Coefficients of determination R~2≥0.998 were achieved for each enantiomer in the cucumber, tomato and soil matrix calibration curves within the range of 0.5-50 μg mL~(-1). The limits of quantification for all enantiomers in three matrices were all below 0.1 μg mL~(-1). The methodology was successfully applied for simultaneous enantioselective analysis of difenoconazole stereoisomers and their metabolite in the real samples, indicating its efficacy in investigating the environmental stereochemistry of difenoconazole in food and environmental matrix.

分类号： O65

  • 相关文献

[1]Assessment and Comparison of in vitro Immunoregulatory Activity of Three Astaxanthin Stereoisomers. Sun Weihong,Xing Lihong,Leng Kailiang,Zhai Yuxiu,Liu Xiaofang,Sun Weihong,Lin Hong. 2016

[2]The application of chiral ultra-high-performance liquid chromatography tandem mass spectrometry to the separation of the zoxamide enantiomers and the study of enantioselective degradation process in agricultural plants. Dong, Fengshou,Chen, Zenglong,Xu, Jun,Liu, Xingang,Wu, Xiaohu,Zheng, Yongquan.

[3]Simultaneous enantioselective determination of isocarbophos and its main metabolite isocarbophos oxon in rice, soil, and water by chiral liquid chromatography and tandem mass spectrometry. Yao, Zhoulin,Lin, Mei,Wang, Tianyu,Ping, Xinliang,Wu, Shaohui,Yao, Zhoulin,Xu, Mingfei,Wang, Qiang,Zhang, Hu,Yao, Zhoulin,Xu, Mingfei,Wang, Qiang,Zhang, Hu,Yao, Zhoulin,Xu, Mingfei,Wang, Qiang,Zhang, Hu,Yao, Zhoulin.

[4]Effects of the bioconcentration and parental transfer of environmentally relevant concentrations of difenoconazole on endocrine disruption in zebrafish (Danio rerio). Teng, Miaomiao,Zhu, Wentao,Wang, Yao,Wang, Dezhen,Wang, Chengju,Qi, Suzhen,Dong, Kai. 2018

[5]Determination of difenoconazole residue in tomato during home canning by UPLC-MS/MS. Dong, Fengshou,Xu, Jun,Liu, Xingang,Li, Jing,Li, Yuanbo,Chen, Xiu,Zheng, Yongquan,Zhang, Changpeng,Shan, Weili. 2012

[6]Concentrations and dissipation of difenoconazole and fluxapyroxad residues in apples and soil, determined by ultrahigh-performance liquid chromatography electrospray ionization tandem mass spectrometry. Jia, Chunhong,Zhao, Ercheng,Chen, Li,Yu, Pingzhong,Jing, Junjie,He, Min,Zheng, Yongquan.

[7]Comparison of Greenhouse and Field Degradation Behaviour of Isoprocarb, Hexaflumuron and Difenoconazole in Perilla frutescens. Sun, Caixia,Zhang, Hu,Tang, Tao,Qian, Mingrong,Yuan, Yuwei,Zhang, Zhiheng,Sun, Caixia,Zhang, Hu,Tang, Tao,Qian, Mingrong,Yuan, Yuwei,Zhang, Zhiheng,Sun, Caixia,Zhang, Hu,Tang, Tao,Qian, Mingrong,Yuan, Yuwei,Zhang, Zhiheng.

[8]Metabolomics and transcriptomics reveal the toxicity of difenoconazole to the early life stages of zebrafish (Danio rerio). Teng, Miaomiao,Zhu, Wentao,Wang, Dezhen,Wang, Yao,Yan, Jin,Zheng, Mingqi,Wang, Chengju,Qi, Suzhen,Dong, Kai. 2018

[9]Residue Determination of Epoxiconazole, Indoxacarb and Difenoconazole in Tea, Tea Infusion and Soil using Ultra High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry. Zhang Xin-Zhong,Luo Feng-Jian,Chen Zong-Mao,Liu Guang-Ming,Lou Zheng-Yun,Wang Fang,Wu Lu-Chao,Zhang Xin-Zhong,Luo Feng-Jian,Chen Zong-Mao,Liu Guang-Ming,Lou Zheng-Yun,Zhang Xin-Zhong,Luo Feng-Jian,Chen Zong-Mao,Liu Guang-Ming,Lou Zheng-Yun. 2013

[10]Sex-specific effects of difenoconazole on the growth hormone endocrine axis in adult zebrafish (Danio rerio). Teng, Miaomiao,Zhu, Wentao,Wang, Yao,Wang, Dezhen,Yang, Yang,Li, Hui,Li, Chenglong,Wang, Chengju,Qi, Suzhen,Dong, Kai. 2017

[11]Dissipation and Residues of Difenoconazole and Azoxystrobin in Bananas and Soil in Two Agro-Climatic Zones of China. Huan, Zhibo,Xu, Zhi,Lv, Daizhu,Xie, Defang,Luo, Jinhui.

[12]Degradation of three fungicides following application on strawberry and a risk assessment of their toxicity under greenhouse conditions. Sun, Caixia,Cang, Tao,Wang, Zhiwei,Wang, Xinquan,Yu, Ruixian,Wang, Qiang,Zhao, Xueping.

[13]The developmental effect of difenoconazole on zebrafish embryos: A mechanism research. Mu, Xiyan,Chai, Tingting,Zhu, Lizhen,Li, Xuefeng,Wang, Chengju,Mu, Xiyan,Huang, Ying,Shen, Gongming,Li, Yingren,Wang, Kai.

[14]Degradation of four fungicides in tropical soils from Hainan, China. Han, Bing Jun,Peng, Li Xu,Chen, Li Xia.

作者其他论文 更多>>

微信小程序